Pål_pic.jpg
Pål_pic.jpg
Institutt for farmasi paal.johnsen@uit.no +4777623156 +47 47688974 Tromsø FARM F4. 218

Johnsen, Pål Jarle


Professor mikrobiologi, Forskningsgruppeleder


  • Christopher Frøhlich, Vidar Sørum, Nobuhiko Tokuriki, Pål Jarle Johnsen, Ørjan Samuelsen :
    Evolution of β-lactamase-mediated cefiderocol resistance
    Journal of Antimicrobial Chemotherapy 2022 ARKIV / DOI
  • Rebecca Ashley Gladstone, Alan McNally, Anna Kaarina Pöntinen, Gerry Tonkin-Hill, John A. Lees, Kusti Onni Skytén m.fl.:
    Emergence and dissemination of antimicrobial resistance in Escherichia coli causing bloodstream infections in Norway in 2002–17: a nationwide, longitudinal, microbial population genomic study
    Lancet Microbe 29. juni 2021 FULLTEKST / ARKIV / DOI
  • Julia Kloos, João A Gama, Joachim Hegstad, Ørjan Samuelsen, Pål J Johnsen :
    Piggybacking on Niche Adaptation Improves the Maintenance of Multidrug-Resistance Plasmids
    Molecular Biology and Evolution (MBE) 2021 ARKIV / DOI
  • Pål Jarle Johnsen, João Alves Gama, Klaus Harms :
    Bacterial evolution on demand
    eLIFE 2021 ARKIV / DOI
  • Sergio Arredondo Alonso, Anna Kaarina Pöntinen, Francois Pierre Alexandre Cleon, Rebecca Ashley Gladstone, Anita C. Schürch, Pål Jarle Johnsen m.fl.:
    A high-throughput multiplexing and selection strategy to complete bacterial genomes
    GigaScience 2021 ARKIV / DOI
  • Macaulay Winter, Angus Buckling, Klaus Harms, Pål Jarle Johnsen, Michiel Vos :
    Antimicrobial resistance acquisition via natural transformation: context is everything
    Current Opinion in Microbiology 2021 ARKIV / DOI
  • Jonina Gudmundsdottir, Elizabeth G. Aarag Fredheim, Catharina I.M. Koumans, Joachim Hegstad, Po-Cheng Tang, Dan I. Andersson m.fl.:
    The chemotherapeutic drug methotrexate selects for antibiotic resistance
    EBioMedicine 2021 ARKIV / DOI
  • Christopher Frøhlich, João Alves Gama, Klaus Harms, Viivi H. A. Hirvonen, Bjarte Aarmo Lund, Marc W. van der Kamp m.fl.:
    Cryptic β-Lactamase Evolution Is Driven by Low β-Lactam Concentrations
    mSphere 2021 ARKIV / DOI
  • Joao Pedro Alves Gama, Julia Maria Kloos, Pål Jarle Johnsen, Ørjan Samuelsen :
    Host dependent maintenance of a blaNDM-1-encoding plasmid in clinical Escherichia coli isolates
    Scientific Reports 2020 ARKIV / DOI
  • Julia Maria Kloos, Joao Pedro Alves Gama, Pål Jarle Johnsen, Ørjan Samuelsen :
    Host dependent maintenance of a blaNDM-1-encoding plasmid in clinical Escherichia coli isolates
    Scientific Reports 2020 DOI
  • Julia Maria Kloos, Pål Jarle Johnsen, Klaus Harms :
    Tn1 transposition in the course of natural transformation enables horizontal antibiotic resistance spread in Acinetobacter baylyi
    Microbiology (Reading) 2020 ARKIV / DOI
  • Christopher Frøhlich, Vidar Sørum, Ane Molden Thomassen, Pål Jarle Johnsen, Hanna-Kirsti S. Leiros, Ørjan Samuelsen :
    OXA-48-Mediated Ceftazidime-Avibactam Resistance Is Associated with Evolutionary Trade-Offs
    mSphere 2019 ARKIV / DOI
  • Nicole L. Podnecky, Elizabeth Aarag, Julia Maria Kloos, Vidar Sørum, Raul Primicerio, Adam Paul Roberts m.fl.:
    Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli.
    Nature Communications 10. september 2018 ARKIV / DOI
  • Nils Fredrik Hülter, Vidar Sørum, Kristina Borch-Pedersen, Mikkel Meyn Liljegren, Ane Utnes, Raul Primicerio m.fl.:
    Costs and benefits of natural transformation in Acinetobacter baylyi
    BMC Microbiology 2017 ARKIV / DOI
  • Luigimaria Borruso, Klaus Harms, Pål Jarle Johnsen, Kaare Magne Nielsen, Lorenzo Brusetti :
    Distribution of class 1 integrons in a highly impacted catchment
    Science of the Total Environment 2016 FULLTEKST / DOI
  • Maria Chiara Di Luca, Vidar Sørum, Irina Starikova, Julia Maria Kloos, Nils Fredrik Hülter, Mohammed Umaer Naseer m.fl.:
    Low biological cost of carbapenemase-encoding plasmids following transfer from Klebsiella pneumoniae to Escherichia coli
    Journal of Antimicrobial Chemotherapy 2016 DOI
  • Jan Engelstädter, Klaus Harms, Pål Jarle Johnsen :
    The evolutionary dynamics of integrons in changing environments
    The ISME Journal 2016 DOI
  • Klaus Harms, Asbjørn Lunnan, Nils Hulter, Tobias Mourier, Lasse Vinner, Cheryl P Andam m.fl.:
    Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms
    Proceedings of the National Academy of Sciences of the United States of America 2016 ARKIV / DOI
  • Amy Mcleman, Pawel Sierocinski, E Hesse, A Buckling, G Perron, Nils Fredrik Hülter m.fl.:
    No effect of natural transformation on the evolution of resistance to bacteriophages in the Acinetobacter baylyi model system.
    Scientific Reports 21. november 2016 ARKIV / DOI
  • Ole Herman Ambur, Jan Engelstädter, Pål Jarle Johnsen, Eric L. Miller, Daniel E. Rozen :
    Steady at the wheel: Conservative sex and the benefits of bacterial transformation
    Philosophical Transactions of the Royal Society of London. Biological Sciences 2016 ARKIV / DOI
  • Ane Live Gunnes Utnes, Vidar Sørum, Nils Fredrik Hülter, Raul Primicerio, Joachim Hegstad, Julia Maria Kloos m.fl.:
    Growth phase-specific evolutionary benefits of natural transformation in Acinetobacter baylyi
    The ISME Journal 2015 DOI
  • Iren Høyland Löhr, Nils Fredrik Hülter, Eva Bernhoff, Pål Jarle Johnsen, Arnfinn Sundsfjord, Mohammed Umaer Naseer :
    Persistence of a pKPN3-like CTX-M-15-encoding IncFIIK plasmid in a Klebsiella pneumonia ST17 host during two years of intestinal colonization
    PLOS ONE 2015 ARKIV / FULLTEKST / DOI
  • Shweta Fulsundar, Klaus Harms, Gøril Eide Flaten, Pål Jarle Johnsen, Balu Ananda Chopade, Kaare Magne Nielsen :
    Gene transfer potential of outer membrane vesicles of Acinetobacter baylyi and effects of stress on vesiculation
    Applied and Environmental Microbiology 2014 ARKIV / DOI
  • Bruce R: Levin, Fernando Baquero, Pål Jarle Johnsen :
    A model-guided analysis and perspective on the evolution and epidemiology of antibiotic resistance and its future
    Current Opinion in Microbiology 2014 DOI
  • Søren Overballe-Petersen, Klaus Harms, Ludovic A.A. Orlando, J. Victor Moreno Mayar, Simon Rasmussen, Tais W. Dahl m.fl.:
    Bacterial natural transformation by highly fragmented and damaged DNA
    Proceedings of the National Academy of Sciences of the United States of America 2013 DOI
  • Irina Starikova, Mohammed Al-Haroni, Guido Werner, Adam P. Roberts, Vidar Sørum, Kåre M. Nielsen m.fl.:
    Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis
    Journal of Antimicrobial Chemotherapy 2013 ARKIV / DOI
  • Kåre M. Nielsen, Jessica Louise Ray, Pål Jarle Johnsen :
    Horizontal gene transfer: the uptake of extracellular DNA in bacteria
    Elsevier 2012
  • Irina Starikova, Klaus Harms, Pål Haugen, Tracy Munthali Lunde, Raul Primicerio, Ørjan Samuelsen m.fl.:
    A Trade-off between the Fitness Cost of Functional Integrases and Long-trem stability of Integrons
    PLoS Pathogens 2012 FULLTEKST / ARKIV / DOI
  • Sara Domingues, Klaus Harms, W. Florian Fricke, Pål Jarle Johnsen, Gabriela J. da Silva, Kåre M. Nielsen :
    Natural Transformation Facilitates Transfer of Transposons, Integrons and Gene Cassettes between Bacterial Species
    PLoS Pathogens 2012 FULLTEKST / ARKIV / DOI
  • Julia Hurler, Ole Alexander Berg, Merete L. Skar, Anne Hilde Conradi, Pål Jarle Johnsen, Natasa Skalko-Basnet :
    Improved burns therapy: Liposomes-in-hydrogel delivery system for mupirocin
    Journal of Pharmaceutical Sciences 2012 DOI
  • Pål jarle Johnsen, JP Townsend, Thomas Bøhn, Gunnar Skov Simonsen, arnfinn Sundsfjord, Kaare Magne Nielsen :
    Retrospective evidence for a biological cost of vancomycin resistance determinants in the absence of glycopeptide selective pressures
    Journal of Antimicrobial Chemotherapy 2011 ARKIV / DOI
  • Julia Maria Kloos, Lea Jänisch, Anna Kaarina Pöntinen, Ørjan Samuelsen, Pål Jarle Johnsen, Jukka Corander m.fl.:
    Different metabolic proficiency among dominant ExPEC lineages
    2022
  • Christopher Frøhlich, Adrian H. Bunzel, Marc W. Van Der Kamp, Adrian J. Mulholland, Hanna-Kirsti Schrøder Leiros, Pål Jarle Johnsen m.fl.:
    Positive epistasis drives the molecular evolution of the carbapenemase OXA-48
    2022
  • João Alves Gama, Julia Maria Kloos, Joachim Hegstad, Ørjan Samuelsen, Pål Jarle Johnsen :
    Plasmid cost alleviation as a consequence of niche-adaptation
    2021
  • Julia Maria Kloos, João Alves Gama, Joachim Hegstad, Ørjan Samuelsen, Pål Jarle Johnsen :
    Plasmid Cost Alleviation By Hitchhiking On Niche-adaptation
    2021
  • Julia Maria Kloos, Joao Pedro Alves Gama, Joachim Hegstad, Ørjan Samuelsen, Pål Jarle Johnsen :
    E. coli Niche Adaptation Improves Plasmid Maintenance
    2021
  • Sybil Akua Okyerewa Obuobi, Kjersti Julin, Elizabeth G. Aarag Fredheim, Pål Jarle Johnsen, Mona Johannessen, Natasa Skalko-Basnet :
    Liposomal delivery of antibiotic loaded DNA nanogels against intracellular infections
    2020
  • Christopher Frøhlich, Hanna-Kirsti S. Leiros, Pål Jarle Johnsen, Ørjan Samuelsen :
    Sub-inhibitory concentrations of ceftazidime drive evolution of the OXA-48 carbapenemase
    2019
  • Christopher Frøhlich, Pål Jarle Johnsen, Hanna-Kirsti Schrøder Leiros, Ørjan Samuelsen :
    Evolution of OXA-48 under sub-MIC conditions
    2019
  • Vidar Sørum, Emma Lu Øynes, Anna Møller, Nicole L. Podnecky, Sören Abel, Ørjan Samuelsen m.fl.:
    Evolutionary stability of collateral susceptibility networks in clinical Escherichia coli strains
    2019
  • Elizabeth G. Aarag Fredheim, Nicole Podnecky, Angie Carola Alarcon Rios, Jessica Nguyen Tran, Chantal Verstappen, Klaus Harms m.fl.:
    Gentamicin collateral sensitivity of ciprofloxacin resistant Escherichia coli is opposed by the rapid development of gentamicin resistance
    2019
  • Vidar Sørum, Anna Sollied Møller, Pål Jarle Johnsen, Ørjan Samuelsen, Sören Abel, Nicole L. Podnecky :
    Evolutionary stability of collateral effects in ciprofloxacin resistant clinical Escherichia coli strains
    2019
  • Vidar Sørum, Anna Sollied Møller, Sören Abel, Ørjan Samuelsen, Nicole L. Podnecky, Pål Jarle Johnsen :
    Evolutionary stability of collateral effects in ciprofloxacin resistant clinical Escherichia coli strains
    2019
  • Julia Maria Kloos, Joao Pedro Alves Gama, Ørjan Samuelsen, Pål Jarle Johnsen :
    Klebsiella pneumoniae MDR-encoding plasmids in clinical isolates of uropathogenic Escherichia coli: plasmid-host co-evolution and its effect on fitness cost of plasmid-mediated resistance
    2018
  • Julia Maria Kloos, Joao Pedro Alves Gama, Ørjan Samuelsen, Pål Jarle Johnsen :
    Plasmid-host co-evolution of Klebsiella pneumoniae MDR-encoding plasmids in clinical isolates of uropathogenic Escherichia coli: effect on fitness cost of plasmid-mediated resistance
    2018
  • Christopher Frøhlich, Vidar Sørum, Hanna-Kirsti S. Leiros, Pål Jarle Johnsen, Ørjan Samuelsen :
    Development of OXA-48 mediated ceftazidme-avibactam resistance is associated with an evolutionary trade-off
    2018
  • Julia Maria Kloos, Kaare Magne Nielsen, Pål Jarle Johnsen, Klaus Harms :
    Natural transformation of Acinetobacter baylyi by transposons
    2017
  • Julia Maria Kloos, Kaare Magne Nielsen, Pål Jarle Johnsen, Klaus Harms :
    Transposition during natural transformation facilitates spread of antibiotic resistance determinants in Acinetobacter baylyi
    2016
  • Klaus Harms, Irina Starikova, Pål Jarle Johnsen :
    Costly Class-1 integrons and the domestication of the the functional integrase
    Mobile Genetic Elements 2013 DOI
  • Pål Jarle Johnsen :
    Is it more to bacterial sex than exploring the fitness landscape of other organisms?
    2012

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    Forskningsinteresser

    I'm head of the Microbial Pharmacology and Population Biology Research Group (MicroPop) and generally interested in bacterial microevolution and focusing on two main topics. 

     

    1. Evolution, selection, and spread of antibiotic resistance: It is clear that the frequency of antimicrobial drug resistance in the community is associated with the total level of antimicrobial consumption. Not so clear is the association between reduced consumption levels and subsequent reduction in the frequency of resistance. We focus on different processes that may counteract the reversal of antimicrobial resistance in drug free environments such as the biological cost of resistance, horizontal gene transfer, and genetic stability of resistance determinants. We are currently expanding our activities to include approaches for optimized antimicrobial treatment informed by principles of evolutionary, and population biology.

    2.    Mechanistic and evolutionary consequences of bacterial recombination:  Horizontal gene transfer (HGT) in bacteria plays a major role in adaptive evolution exemplified by the evolution, spread and selection for antibiotic resistance determinants.  For two out of three described mechanisms of HGT, conjugation and transduction, gene recruitment through recombination is likely a coincidental byproduct of plasmids’ and phages’ need for continuous transmission to new hosts. The third mechanism of HGT, natural transformation, is a complex mechanism for DNA uptake that requires the concerted action of many chromosomal genes. On first consideration it appears evident that this mechanism for HGT has evolved to “sample the available sequence space” in order to increase the adaptation rates of transformable strains. However, other hypotheses exist for both the evolution and the maintenance of natural transformation in bacteria. We are currently testing a number of these hypotheses both experimentally and theoretically. We are also interested in the mechanistic basis of bacterial recombination and it affects genetic diversity in microbes.

    CV AND CURRENT POSITION

    2000-2004. 
Ph.D. Student
Supervisors Prof. Arnfinn Sundsfjord, Dr. Med. Gunnar Skov Simonsen

    2004-2005
. Post Doctoral Fellowship. Department of Pharmacology. Prof. Kaare M. Nielsen Lab, Institute of Pharmacy, University of Tromsø, Norway.

    2006-2008: Personal postdoc grant The Norwegian Research Council (NFR), including:

    2007-2008 
Visiting Post Doc. Bruce Levin Lab. Emory University, Atlanta, USA

    2008- Associate Professor of Microbiology. Research group for Microbiology, pharmaco- and molecular epidemiology, Department of Pharmacy, University of Tromsø. Group leader: Prof. Kaare M. Nielsen.

    2014- Associate Professor, Group Leader Microbial Pharmacology and Population Biology Research Group 

     

    2015: Full Professor, Group Leader Microbial Pharmacology and Population Biology Research Group 

     

    SELECTED PUBLICATIONS 

     

    1. Podnecky NL, Fredheim EGA,, Kloos J, Sørum V, Primicerio R, Roberts AP, Rozen DE, Samuelsen Ø, PJ Johnsen. Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli. Nat. Commun. 2018 9: 3673

    2.  Di Luca MC., Sørum V., Starikova I., Klos J., Hülter N., Naseer U., Johnsen PJ., Samuelsen Ø. Low biological cost of carbapenemase producing plasmids following transfer from Klebsiella pneumonia to Escherichia coli. J Antimicrob Chemother. 2017 Jan;72(1):85-89. 

    3.  Harms K., Lunnan A., Hülter N., Mourier T., Vinner L., Andam CP., Martiinen P., Fridholm H., Hansen AJ., Hanage WP., Nielsen KM., Willerslev E., PJ. Johnsen. Substitutions of short Heterologous DNA segements of intragenomic or extragenomic origins produce clustered genomic polymorphisms. Proc Nat Acad Sci U S A, 2016 113:52 15066-071

    4. Ambur OH., Engelstädter J., Johnsen PJ., Miller EL., Rozen DE. Steady at the wheel: conservative sex and the benefits of natural transformation. Philos Trans R Soc Lond B Biol Sci. 2016 Oct 19;371(1706). pii: 20150528.
    5. Di Luca MC., Sørum V., Starikova I., Klos J., Hülter N., Naseer U., Johnsen PJ., Samuelsen Ø. Low biological cost of carbapenemase producing plasmids following transfer from Klebsiella pneumonia to Escherichia coli. J Antimicrob Chemother. 2016 Sep 2. pii: dkw350
    6. Engelstädter J., Harms K., Johnsen PJ. The evolutionary dynamics of integrons in changing environments ISME J, 2016, Jun;10(6):1296-307.
    7. Utnes A., Sorum V., Hulter N., Primicerio R., Kloos J., Nielsen K.M., Johnsen PJ. Growth phase specific evolutionary benefits of natural transformation in Acinetobacter baylyi.  ISME J, 2015;Oct;9(10):2221-31. 
    8. Levin B. R., Baquero F., Johnsen PJ. A Model-Guided Analysis and Perspective on the Evolution and Epidemiology of Antibiotic Resistance and its Future. Invited Current Opin Microbiol. 2014: Jun;19:83-9
    9. Overballe-Petersen S., Harms K*., Orlando L. A., Mayar J. V., Rasmussen S., Dahl T. W., Rosing M. T., Poole A. M., Sicheritz-Ponten T., Brunak S., Inselmann S., de Vries J., Wackernagel W., Pybus O. G., Nielsen R., Johnsen P. J., Nielsen K. M., and Willerslev E. Bacterial natural transformation by highly fragmented and damaged DNA. Proc Natl Acad Sci U S A. 2013: 110(49):19860-5. doi: 10.1073/pnas.1315278110. Epub 2013 Nov 18.
    10. Starikova I., Al-Haroni M., Werner G., Roberts A. P., Sørum V., Nielsen K.M., Johnsen PJ. Fitness costs of mobile genetic elements in Enterococcus faecium and Enterococcus faecalis.J Antimicrob Chemother. 2013 Dec;68(12):2755-65. Epub 2013 Jul 5.
    11. Harms K., Starikova I., Johnsen PJ. Costly class 1 integrons and the domesticatioon of the functional integrase. Mob Genet Elements. 2013 Mar 1;3(2):e24774
    12. StarikovaI., Harms K., Lunde T. M., Haugen P., Primicerio R., Samuelsen Ø., Nielsen K. M, Johnsen PJ. 2012. A trade-off between the fitness cost of functional integrases and long-term stability of integrons. PloS Pathog.8(11):e1003043.
    13. Domingues S., Harms K., Fricke F. W., Johnsen P. J., da Silva G., and K. M. Nielsen. 2012. Natural Transformation Facilitates Transfer of Transposons, Integrons and Gene Cassettes between Bacterial Species. PloS Pathog. 8 (8): e1002837
    14. Johnsen PJ., Townsend J. P., Bøhn T., Simonsen G. S., Sundsfjord A., and Nielsen K. M. 2011. Retrospective evidence for a biolog10ical cost of vancomycin resistance in Enterococcus faeciumJ. Antimicrobial Agents and Chemother. 66(3):608-10
    15. Johnsen PJ., and Levin B. R.  2010. Adjusting to alien genes. Mol Microbiol. 75: 1061-63 Invited commentary
    16. Ray, J. L., Harms K., O. G. Wikmark, Starikova I., Johnsen PJ Nielsen KM. 2009. Sexual isolation in Acinetobacter baylyi is locus-specific and varies 10 000-fold over the genome. Genetics. 182: 1165-81.
    17. Johnsen PJ., Townsend J., Bohn T., Simonsen GS., Sundsfjord A., Nielsen KM. 2009. Factors affecting the reversal of antimicrobial resistance. Lancet Infect. Dis. 9: 357-364.
    18. Johnsen PJ., Dubnau D., Levin BR. Episodic Selection and the Maintenance of Competence and Transformation in Bacillus subtilis. 2009. Genetics. 181: 1521-1533. Chosen as Editorial highlight for April 2009



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