Time-gated coherent anti-Stokes Raman scattering (T-CARS) microscopy
Thomas Huser will give a lecture Thursday 5th March at the Dept. of Chemistry: C321 in Realfagsbygget at 13:15. Physics-people are also invited.
Thomas Huser
NSF Center for Biophotonics Science and Technology, and Department of Internal Medicine University of California, Davis 2700 Stockton Blvd, Suite 1400 Sacramento, CA 95758
Email: trhuser@ucdavis.edu
Time-resolved coherent anti-Stokes Raman scattering (CARS) microscopy provides an elegant means to extract a maximum amount of information from unlabeled biological samples. By combining time-resolved single photon counting (TCSPC) schemes with CARS microscopy, we are able to separate the arrival time of CARS photons from those of other sources, e.g. 2-photon excited fluorescence. The use of special collection optics also enables the use of TCSPC to distinguish between forward and back-reflected CARS signals. We demonstrate this by imaging the formation of lipid droplets in living human monocytes and hepatocytes after treatment with lipolysis products. I will also discuss advanced novel forms of four-wave mixing microscopy, which enable us to further improve the sensitivity and selectivity of nonlinear vibrational microscopy.
