Peter McCourt
Professor
- Szafranska, Karolina; Neuman, Tanja; Baster, Zbigniew; Rajfur, Zenon; Szelest, Oskar; Holte, Christopher Florian; Kubisiak, Agata; Kus, Edyta; Wolfson, Deanna; Chlopicki, Stefan; Ahluwalia, Balpreet Singh; Lekka, Malgorzata; Szymonski, Marek; McCourt, Peter Anthony; Zapotoczny, Barlomiej. From fixed-dried to wet-fixed to live-comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations. Nanophotonics 2022. ISSN 2192-8606.s doi: 10.1515/nanoph-2021-0818.
- Hunt, Nicholas J; McCourt, Peter Anthony; Kuncic, Zdenka; Le Couteur, David G; Cogger, Victoria C. Opportunities and Challenges for Nanotherapeutics for the Aging Population. Frontiers in Nanotechnology 2022; Volum 4. ISSN 2673-3013.s doi: 10.3389/fnano.2022.832524.
- Butola, Ankit; Coucheron, David Andre; Szafranska, Karolina; Ahmad, Azeem; Mao, Hong; Tinguely, Jean-Claude; McCourt, Peter Anthony; Paramasivam, Senthilkumaran; Mehta, Dalip Singh; Agarwal, Krishna; Ahluwalia, Balpreet Singh. Multimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells. (fulltekst) Proceedings of the National Academy of Sciences of the United States of America 2021; Volum 118 (47). ISSN 0027-8424.s doi: 10.1073/pnas.2115323118.
- Szafranska, Karolina; Holte, Christopher Florian; Kruse, Larissa Dorothea; Mao, Hong; Øie, Cristina Ionica; Symonski, Marek; Zapotoczny, Bartlomiej; McCourt, Peter Anthony. Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study. Micron 2021; Volum 150. ISSN 0968-4328.s 103121 - .s doi: 10.1016/j.micron.2021.103121.
- Larsen, Anett Kristin; Simon-Santamaria, Jaione; Elvevold, kjetil helge; Ericzon, Bo Gøran; Mortensen, Kim Erlend; McCourt, Peter Anthony; Smedsrød, Bård; Sørensen, Karen Kristine. Autofluorescence in freshly isolated adult human liver sinusoidal cells. European journal of histochemistry 2021; Volum 65 (4). ISSN 1121-760X.s doi: 10.4081/ejh.2021.3337.
- Bhandari, Sabin; Larsen, Anett Kristin; McCourt, Peter Anthony; Smedsrød, Bård; Sørensen, Karen Kristine. The scavenger function of liver sinusoidal endothelial cells in health and disease. Frontiers in Physiology 2021; Volum 12. ISSN 1664-042X.s doi: 10.3389/fphys.2021.757469.
- Szafranska, Karolina; Kruse, Larissa Dorothea; Holte, Christopher Florian; McCourt, Peter Anthony; Zapotoczny, Bartlomiej. The wHole Story About Fenestrations in LSEC. Frontiers in Physiology 2021; Volum 12. ISSN 1664-042X.s doi: 10.3389/fphys.2021.735573.
- Hunt, Nicholas J.; Lockwood, Glen P; Kang, Sun W. S.; Westwood, Lara; Limantoro, Christina; Chrzanowski, Wojciech; McCourt, Peter Anthony; Kuncic, Zdenka; Le Couteur, David G; Cogger, Victoria C.. Quantum Dot Nanomedicine Formulations Dramatically Improve Pharmacological Properties and Alter Uptake Pathways of Metformin and Nicotinamide Mononucleotide in Aging Mice. ACS Nano 2021; Volum 15. ISSN 1936-0851.s 4710 - 4727.s doi: 10.1021/acsnano.0c09278.
- Tinguely, Jean-Claude; Anna Maria, Steyer; Øie, Cristina Ionica; Helle, Øystein Ivar; Dullo, Firehun Tsige; Olsen, Randi; McCourt, Peter; Schwab, Yannick; Ahluwalia, Balpreet Singh. Photonic-chip assisted correlative light and electron microscopy. Communications Biology 2020. ISSN 2399-3642.s doi: 10.1038/S42003-020-01473-4.
- Hunt, Nicholas J; Lockwood, Glen P; Le Couteur, Frank H.; McCourt, Peter Anthony; Singla, Nidhi; Kang, Sun; Burgess, Andrew; Kuncic, Zdenka; Le Couteur, David G; Cogger, Victoria C. Rapid Intestinal Uptake and Targeted Delivery to the Liver Endothelium Using Orally Administered Silver Sulfide Quantum Dots.. ACS Nano 2020; Volum 14. ISSN 1936-0851.s doi: 10.1021/acsnano.9b06071.
- Øie, Cristina Ionica; Wolfson, Deanna; Yasunori, Tanji; Dumitriu, Gianina; Sørensen, Karen Kristine; McCourt, Peter Anthony; Ahluwalia, Balpreet Singh; Smedsrød, Bård. Liver sinusoidal endothelial cells contribute to the uptake and degradation of entero bacterial viruses.. Scientific Reports 2020. ISSN 2045-2322.s doi: 10.1038/s41598-020-57652-0.
- Bhandari, Sabin; Li, Ruomei; Simon-Santamaria, Jaione; McCourt, Peter Anthony; Johansen, Steinar Daae; Smedsrød, Bård; Martinez, Inigo Zubiavrre; Sørensen, Karen Kristine. Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages. BMC Molecular and Cell Biology 2020; Volum 21. ISSN 2661-8850.s doi: 10.1186/s12860-020-00331-9.
- Boninsegna, Matteo; Jayakumar, Nikhil; Dullo, Firehun Tsige; McCourt, Peter Anthony; Huser, Thomas Rolf; Ahluwalia, Balpreet Singh; Corbera, Antoni Homs. Waveguide chip coupled with microfluidics enables super-resolution live-cell imaging. Proceedings of SPIE, the International Society for Optical Engineering 2020. ISSN 0277-786X.s doi: 10.1117/12.2568385.
- Tinguely, Jean-Claude; Anna Maria, Steyer; Øie, Cristina Ionica; Helle, Øystein Ivar; Dullo, Firehun Tsige; Olsen, Randi; McCourt, Peter Anthony; Schwab, Yannick; Ahluwalia, Balpreet Singh. Photonic-chip assisted corelative light and electron microscopy. arXiv.org 2019. ISSN 2331-8422.
- Hunt, Nicholas J; Lockwood, Glen P; Warren, Alessandra; Mao, Hong; McCourt, Peter Anthony; Le Couteur, David George; Cogger, Victoria Carroll. Manipulating fenestrations in young and old liver sinusoidal endothelial cells. American Journal of Physiology - Gastrointestinal and Liver Physiology 2019; Volum 316 (1). ISSN 0193-1857.s G144 - G154.s doi: 10.1152/ajpgi.00179.2018.
- Hunt, Nick; Lockwood, Glen P; Kang, Sun; Pupitel, Tamara; Clark, Ximonie; Mao, Hong; McCourt, Peter Anthony; Cooney, Greg; Wali, Jibran; Le Couteur, Frank; Le Couteur, David George; Cogger, Victoria Carroll. The effects of metformin on age-related changes in the liver sinusoidal endothelial cell. The journals of gerontology. Series A, Biological sciences and medical sciences 2019. ISSN 1079-5006.s doi: 10.1093/gerona/glz153.
- Mao, Hong; Diekmann, Robin; Liang, Hai Po H; Cogger, Victoria Carroll; Le Couteur, David George; Lockwood, Glen P; Hunt, Nick; Schuttpelz, Mark; Huser, Thomas Rolf; Chen, Vivien; McCourt, Peter Anthony. Cost-efficient nanoscopy reveals nanoscale architecture of liver cells and platelets. Nanophotonics 2019; Volum 8 (7). ISSN 2192-8606.s 1299 - 1313.s doi: 10.1515/nanoph-2019-0066.
- Øie, Cristina Ionica; Mönkemöller, Viola; Hubner, Wolfgang; Schuttpelz, Mark; Mao, Hong; Ahluwalia, Balpreet Singh; Huser, Thomas Rolf; McCourt, Peter Anthony. New ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrations. (fulltekst) Nanophotonics 2018. ISSN 2192-8606.s doi: 10.1515/nanoph-2017-0055.
- Hunt, Nicholas J; McCourt, Peter Anthony; Le Couteur, David George; Cogger, Victoria C. Novel targets for delaying aging: The importance of the liver and advances in drug delivery. Advanced Drug Delivery Reviews 2018. ISSN 0169-409X.s doi: 10.1016/j.addr.2018.09.006.
- Diekmann, Robin; Helle, Øystein Ivar; Øie, Cristina Ionica; McCourt, Peter Anthony; Huser, Thomas Rolf; Schuttpelz, Mark; Ahluwalia, Balpreet Singh. Chip-based wide field-of-view nanoscopy. Nature Photonics 2017. ISSN 1749-4885.s doi: 10.1038/nphoton.2017.55.
- McCourt, Peter Anthony; Oteiza, Ana; Cao, Ben; Nilsson, Susie. Isolation of murine bone marrow scavenging sinusoidal endothelial cells.. Methods in molecular biology 2015; Volum 1235. ISSN 1064-3745.s 59 - 71.s doi: 10.1007/978-1-4939-1785-3_6.
- Ahluwalia, Balpreet Singh; McCourt, Peter Anthony; Oteiza, Ana; Wilkinson, James S.; Huser, Thomas Rolf; Hellesø, Olav Gaute. Squeezing red blood cells on an optical waveguide to monitor cell deformability during blood storage. The Analyst 2015; Volum 140 (1). ISSN 0003-2654.s 223 - 229.s doi: 10.1039/c4an01181c.
- Mönkemöller, Viola; Øie, Cristina Ionica; Hubner, Wolfgang; Huser, Thomas Rolf; McCourt, Peter Anthony. Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Scientific Reports 2015; Volum 5. ISSN 2045-2322.s doi: 10.1038/srep16279.
- Helle, Øystein Ivar; Øie, Cristina Ionica; McCourt, Peter Anthony; Ahluwalia, Balpreet Singh. Chip-based optical microscopy for imaging membrane sieve plates of liver scavenger cells. Proceedings of SPIE, the International Society for Optical Engineering 2015; Volum 9554. ISSN 0277-786X.s doi: 10.1117/12.2187277.
- Ahluwalia, Balpreet Singh; McCourt, Peter Anthony; Wilkinson, James S.; Huser, Thomas; Hellesø, Olav Gaute. Optical deformation of red blood cells trapped on a narrow waveguide. SPIE - International Society for Optical Engineering 2014 ISBN 9781628411911.s doi: 10.1117/12.2062590.
- Ahluwalia, Balpreet Singh; McCourt, Peter Anthony; Oteiza, Ana; Wilkinson, James S.; Huser, Thomas Rolf; Hellesø, Olav Gaute. Squeezing red blood cells with the evanescent field of an optical waveguide to monitor cell deformability during blood storage. The Analyst 2014; Volum 140. ISSN 0003-2654.s 223 - 229.s doi: 10.1039/C4AN01181C.
- Simon-Santamaria, Jaione; Rinaldo, Christine Hanssen; Kardas, Piotr; Li, Ruomei; Malovic, Ivana; Elvevold, kjetil helge; McCourt, Peter Anthony; Smedsrød, Bård; Hirsch, Hans H.; Sørensen, Karen Kristine. Efficient uptake of blood-borne BK and JC polyomavirus-like particles in endothelial cells of liver sinusoids and renal vasa recta. PLOS ONE 2014; Volum 9 (11). ISSN 1932-6203.s doi: 10.1371/journal.pone.0111762.
- Mönkemöller, Viola; Schuttpelz, Mark; McCourt, Peter Anthony; Sørensen, Karen Kristine; Smedsrød, Bård; Huser, Thomas Rolf. Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry, Chemical Physics - PCCP 2014; Volum 16 (24). ISSN 1463-9076.s 12576 - 12581.s doi: 10.1039/c4cp01574f.
- Svistounov, Dmitri; Oteiza, Ana; Zykova, Svetlana; Sørensen, Karen Kristine; McCourt, Peter Anthony; McLachlan, Andrew J; Mccuskey, Robert Scott; Smedsrød, Bård. Hepatic disposal of advanced glycation end products during maturation and aging. Experimental Gerontology 2013; Volum 48 (6). ISSN 0531-5565.s 549 - 556.s doi: 10.1016/j.exger.2013.03.005.
- Sørensen, Karen Kristine; McCourt, Peter Anthony; Berg, Trond; Crossley, Clive; LeCouteur, David; Wake, Kenjiro; Smedsrød, Bård. The scavenger endothelial cell - a new player in homeostasis and immunity. American Journal of Physiology. Regulatory Integrative and Comparative Physiology 2012; Volum 303 (12). ISSN 0363-6119.s R1217 - R1230.s doi: 10.1152/ajpregu.00686.2011.
- Li, Ruomei; Oteiza, Ana; Sørensen, Karen; Mccourt, Peter Anthony; Olsen, Randi; Smedsrød, Bård; Svistounov, Dmitri. Role of liver sinusoidal endothelial cells and stabilins in elimination of oxidized low-density lipoproteins. American Journal of Physiology - Gastrointestinal and Liver Physiology 2011; Volum 300 (1). ISSN 0193-1857.s G71 - G81.s doi: 10.1152/ajpgi.00215.2010.
- Løvhaugen, Pål; Ahluwalia, Balpreet Singh; Huser, Thomas Rolf; Mccourt, Peter Anthony; Hellesø, Olav Gaute. Optical trapping forces on biological cells on a waveguide surface. Proceedings of SPIE, the International Society for Optical Engineering 2011; Volum 7902. ISSN 0277-786X.s doi: 10.1117/12.873779.
- Simon, Jaione; Malovic, Ivana; Warren, Alessandra; Oteiza, Ana; LeCouteur, David; Smedsrød, Bård; Mccourt, Peter Anthony; Sørensen, Karen Kristine. Age-Related Changes in Scavenger Receptor-Mediated Endocytosis in Rat Liver Sinusoidal Endothelial Cells. The journals of gerontology. Series A, Biological sciences and medical sciences 2010; Volum 65 (9). ISSN 1079-5006.s 951 - 960.s doi: 10.1093/gerona/glq108.
- Cogger, V.C.; McNerney, Gregory P; Nyunt, Tun; DeLeve, Laurie D; Mccourt, Peter Anthony; Smedsrød, Bård; Le Couteur, D.G.; Huser, Thomas Rolf. Three-dimensional structured illumination microscopy of liver sinusoidal endothelial cell fenestrations. Journal of Structural Biology 2010; Volum 171 (3). ISSN 1047-8477.s 382 - 388.s doi: 10.1016/j.jsb.2010.06.001.
- Ahluwalia, Balpreet Singh; Mccourt, Peter Anthony; Huser, Thomas Rolf; Hellesø, Olav Gaute. Optical trapping and propulsion of red blood cells on waveguide surfaces. Optics Express 2010; Volum 18 (20). ISSN 1094-4087.s 21053 - 21061.
- Nedredal, Geir Ivar; Elvevold, kjetil helge; Ytrebø, Lars Marius; Fuskevåg, Ole Martin; Pettersen, Ingvild; Mccourt, Peter Anthony; Bertheussen, Kjell; Smedsrød, Bård; Revhaug, Arthur. Porcine Liver Sinusoidal Endothelial Cells Contribute Significantly to Intrahepatic Ammonia Metabolism. Hepatology 2009; Volum 50 (3). ISSN 0270-9139.s 900 - 908.s doi: 10.1002/hep.23076.
- Qian, Hong; Johansson, Sophie; Mccourt, Peter Anthony; Smedsrød, Bård; Ekblom, Marja; Johansson, Staffan. Stabilins are expressed in bone marrow sinusoidal endothelial cells and mediate scavenging and cell adhesive functions. Biochemical and Biophysical Research Communications - BBRC 2009; Volum 390. ISSN 0006-291X.s 883 - 886.s doi: 10.1016/j.bbrc.2009.10.068.
- Li, Ruomei; Mccourt, Peter Anthony; Schledzewski, Kai; Goerdt, Sergij; Moldenhauer, Gerhard; Liu, xipu; Smedsrød, Bård; Sørensen, Karen. Endocytosis of Advanced Glycation End-Products in Bovine Choriocapillaris Endothelial Cells. Microcirculation 2009; Volum 16 (7). ISSN 1073-9688.s 640 - 655.s doi: 10.1080/10739680903133185.
- Elvevold, kjetil helge; Simon, Jaione; Hasvold, hege; Mccourt, Peter Anthony; Smedsrød, Bård; Sørensen, Karen. Liver Sinusoidal Endothelial Cells Depend on Mannose Receptor-Mediated Recruitment of Lysosomal Enzymes for Normal Degradation Capacity. Hepatology 2008; Volum 48 (6). ISSN 0270-9139.s 2007 - 2015.s doi: 10.1002/hep.22527.
- Malovic, Ivana; Sørensen, Karen; Elvevold, kjetil helge; Nedredal, Geir Ivar; Paulsen, steinar; Erofeev, Alexander V.; Smedsrød, Bård; Mccourt, Peter Anthony. The mannose receptor on murine liver sinusoidal endothelial cells is the main denatured collagen clearance receptor. Hepatology 2007; Volum 45. ISSN 0270-9139.s 1454 - 1461.s doi: 10.1002/hep.21639.
- Yoshida, M; Nishikawa, Y; Omori, Y; Yoshioka, T; Tokairin, T; Mccourt, Peter Anthony; Enomoto, K. Involvement of signaling of VEGF and TGF-beta in differentiation of sinusoidal endothelial cells during culture of fetal rat liver cells. Cell and Tissue Research 2007; Volum 329. ISSN 0302-766X.s doi: 10.1007/s00441-007-0387-5.
- Hansen, B; Longati, P.; Elvevold, Kjetil Helge; Nedredal, Geir Ivar; Schledzewski, K.; Olsen, Randi; Falkowski, Martin; Kzhyshkowska, J.; Carlsson, F.; Johansson, S.; Smedsrød, Bård; Goerdt, S.; Johansson, S; Mccourt, Peter Anthony. Stabilin-1 and stabilin-2 are both directed into the early endocytic pathway in hepatic sinusoidal endothelium via interactions with clathrin/AP-2, independent of ligand binding. Experimental Cell Research 2005; Volum 303(1). ISSN 0014-4827.s 160 - 173.
- McCourt, Peter Anthony; Hansen, B; Svistounov, Dmitri; Johansson, S.; Longati, P.; Schledzewski, K; Kzhyshkowska, J.; Goerdt, S; Johansson, Sophie; Smedsrød, Bård. The liver sinusoidal endothelial cell hyaluronan receptor and its homolog, stabilin-1 - their roles (known and unknown) in endocytosis. 2005 ISBN 0-9771359-0-X.s 611 - 614.
- Svistounov, Dmitri; Berg, Tore J.; McCourt, Peter Anthony; Zykova, Svetlana; Elvevold, Kjetil Helge; Nagai, Ryoji; Horiuchi, Seikoh; Smedsrød, Bård. Lack of recognition of N-epsilon-(carboxymethyl)lysine by the mouse liver reticulo-endothelial system: implications for pathophysiology. Biochemical and Biophysical Research Communications - BBRC 2003; Volum 309. ISSN 0006-291X.s 786 - 791.
- Politz, Oliver; Gratchev, Alexei; McCourt, Peter Anthony; Schledzewski, Kai; Guillot, Pierre; Johansson, Sophie; Svineng, Gunbjørg; Franke, Paul; Kannicht, Catherine; Welten, Florian; Johansson, Staffan; Goerdt, Sergej. Stabilin-1 and -2 constitute a novel family of fasciclin-like hyaluronan receptor homologues. Biochemical Journal 2002; Volum 362. ISSN 0264-6021.s 155 - 164.
- McCourt, Peter Anthony. How does the hyaluronan scrap-yard operate?. Matrix Biology 1999; Volum 18. ISSN 0945-053X.s 427 - 432.
- McCourt, Peter Anthony; Smedsrød, Bård; Melkko, Jukka; Johansson, Staffan. Characterisation of an hyaluronan receptor on rat liver endothelial cells and its functional relationship to scavenger receptors. Hepatology 1999; Volum 30. ISSN 0270-9139.s 1276 - 1286.
- McCourt, Peter Anthony; Gustafson, Stefan. On the adsorption of hyaluronan and ICAM-1 to modified hydrophobic resins. International Journal of Biochemistry and Cell Biology 1997; Volum 29. ISSN 1357-2725.s 1179 - 1189.
- Fuxe, Kjell; Agnati, Luigi; Tinner, Barbro; Forsberg, Nina; McCourt, Peter Anthony; Gustafson, Stefan. Evidence for receptors for hyaluronan in discrete nerve cell populations of the brain. Brain Research 1996; Volum 736. ISSN 0006-8993.s 329 - 337.
- Gustafson, Stefan; Forsberg, Nina; McCourt, Peter Anthony; Wikström, Tarja; Björkman, Thomas; Lilja, Kajsa. First International Workshop on hyaluronan in drug delivery: Characterization and localization of the hyaluronan receptor originally found on liver endothelial cells. Royal Society of Medicine. Round Table Series 1994; Volum 33. ISSN 0268-3091.s 43 - 59.
- McCourt, Peter Anthony; Ek, Bo; Forsberg, Nina; Gustafson, Stefan. Intercellular adhesion molecule-1 is a cell surface receptor for hyaluronan. Journal of Biological Chemistry 1994; Volum 269. ISSN 0021-9258.s 30081 - 30084.
- Ashton, Lesley; Brooks, Doug; McCourt, Peter Anthony; Müller, Viviane; Clements, Peter; Hopwood, John. Immunoquantification and enzyme kinetics of a-L-iduronidase: in cultured fibroblasts from mucopolysaccharidosis type I patients. American Journal of Human Genetics 1992; Volum 50. ISSN 0002-9297.s 787 - 794.
- Brooks, Doug; Harper, Greg; Gibson, Gary; Ashton, Lesley; Taylor, Jacquie; McCourt, Peter Anthony; Freeman, Craig; Clements, Peter; Hoffmann, John; Hopwood, John. Hurler syndrome: a patient with abnormally high levels of a-L-iduronidase protein. Biochemical Medicine and Metabolic Biology 1992; Volum 47. ISSN 0885-4505.s 211 - 220.
- Brooks, Doug; Gibson, Gary; McCourt, Peter Anthony; Hopwood, John. A specific fluorogenic assay for N-acetylglucosamine 4-sulphatase activity using immunoadsorption. The Journal of Inherited Metabolic Disease (JIMD) 1991; Volum 14. ISSN 0141-8955.s 5 - 12.
- Brooks, Doug; McCourt, Peter Anthony; Gibson, Gary; Ashton, Lesley; Shutter, Merron; Hopwood, John. Analysis of N-acetylglucosamine-4-sulfatase protein and kinetics in mucopolysaccharidosis type VI patients. American Journal of Human Genetics 1991; Volum 48. ISSN 0002-9297.s 710 - 719.
- Brooks, Doug; McCourt, Peter Anthony; Gibson, Gary; Hopwood, John. Immunoquantification of the low abundance lysosomal enzyme N-acetylgalactosamine 4-sulphatase. The Journal of Inherited Metabolic Disease (JIMD) 1990; Volum 13. ISSN 0141-8955.s 108 - 120.
- Clements, Peter; Brooks, Doug; McCourt, Peter Anthony; Hopwood, John. Immunopurification and characterisation of human a-L-iduronidase with the use of monoclonal antibodies. Biochemical Journal 1987; Volum 259. ISSN 0264-6021.s 199 - 208.
Forskningsinteresser
Studerer reseptorer som er ansvarlige for fjerning av hyaluronan, denaturert kollagen og andre molekyler fra sirkulasjonen via lever sinusoidale endotelceller (LSEC) og beinmarg sinusoidale endotelceller (BMSEC).
Medlem i forskningsgruppe
CV
Name: Peter Anthony Gerard McCourt
Date of Birth: 09.08.1965
Nationality: Australian
Academic background:
1986 – B.Sc. (Honours 1a) Flinders University, Australia
1999 – Ph.D. (Biochemistry), University of Uppsala, Sweden
Areas of research expertise:
1. Protein purification and analysis;
2. Antibody production;
3. Biochemical assays of cytochrome P450 enzymes in hepatocytes
4. Tissue culture;
5. Recombinant protein expression in mammalian and bacterial systems;
6. Bioinformatic, PCR and cloning strategies;
7. Development of clinical ELISAs and other immunoassays.
8. Immunohistochemistry and immuno electron microscopy
9. Isolation of liver sinusoidal endothelial cells (LSEC);
10. Isolation of bone marrow sinusoidal endothelial cells (BMSEC), bone marrow stem cells, homing and engraftment assays.
11. Super resolution optical microscopy methods (e.g. SIM, dSTORM) for use in the study of LSEC fenestrations.
Teaching:
Approved teaching portfolio (a compulsory requirement for teachers at the University of Tromsø) 19.06.12.
Currently teach Biochemistry to Medical Laboratory Scientists and Dental Nurses.
Research background:
2020-present: Professor of Biochemistry, IMB, studying the effects of ageing on LSEC morphology and biochemistry.
2016-2017: Sabbatical at the ANZAC Research Institute (Sydney, Australia), Biogerontology Research Group (hosts: Professors David Le Couteur and Victoria Cogger).
2016-present: Honorary Affiliate of the University of Sydney Medical School (Faculty of Medicine).
2012-2020: Associate Professor, IMB, studying the effects of ageing on LSEC morphology and biochemistry.
2010-11: Guest Researcher, Niche Group, CSIRO Materials Science and Engineering, Clayton, Australia, with Associate Professor Susie Nilsson, studying bone-marrow sinusoidal endothelial cells and stem cells and their utility in bone-marrow transplants.
1999-2012: Post-Doctoral Research Fellow/Research Scientist at the Department of Cell Biology and Histology, Institute for Medical Biology, University of Tromsø with Professor Bård Smedsrød, studying receptors responsible for the clearance of hyaluronan, denatured collagen and other waste connective tissue molecules from the circulation by LSEC.
1993-1999: Ph.D. Student, Department of Medical Biochemistry and Microbiology, University of Uppsala, Sweden under the supervision of Professors Torvard Laurent and Staffan Johansson, working on the purification, characterisation and cloning of hyaluronan/scavenger receptors on liver scavenger endothelial cells.
1990-1993: Guest Researcher, Department of Medical and Physiological Chemistry, University of Uppsala, Sweden under the supervision of Professor Torvard Laurent, studying hyaluronan-binding properties of the monocytic cell lines U937 and J774.
1987-1990: Research Assistant, Lysosomal Diseases Research Unit, Adelaide Children's Hospital, Adelaide, Australia with Professor John Hopwood, working on the purification of lysosomal enzymes, production of hybridoma cell lines/monoclonal antibodies, and development of ELISAs for clinical assessment of lysosomal enzyme deficiencies.
Financial Support:
University of Tromsø Researcher Fellowship, HelseNord, various small foundations, Norwegian Research Council Post-doctoral Fellowship, Tromsø Research Foundation, EU:Horizon 2020 MSCA.