Karolina Szafranska
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Forskningsinteresser
Currently working in a project focusing on the application of various types of super-resolution microscopy for studying fenestrations in liver sinusoidal endothelial cells.
Research methods:
isolation of primary liver cells
cell culture
immunostaining
microscopy - SEM, SIM, STED, STORM, AFM, confocal fluorescent microscopy
correlative microscopy techniques
image analysis, machine learning (ImageJ/Fiji, Ilastik)
Previous projects: MSCA ITN DeLIVER
Undervisning
MBI-3012 Advanced methods in experimental biomedicine
Supervision of master and bachelor students of Biomedicine
Medlem i forskningsgruppe
Medlem i prosjekt
CV
Name: Karolina Joanna Szafranska
Date of birth: 14.09.1993
Nationality: Polish
Academic background:
2016 B.Sc. in Biophysics (Medical Physics) Jagiellonian University, Kraków, Poland, supervisor Marek Szymoński, thesis: Investigation of the morphology of the liver sinusoidal endothelial cells (LSECs) by atomic force microscopy (AFM) and fluorescent microscopy
2017 M.Sc. in Biophysics (Molecular Biophysics) Jagiellonian University, Kraków, Poland, supervisor Marek Szymoński, thesis: Structure of fenestrations and nanomechanical properties of liver sinusoidal endothelial cells altered by drugs and toxins
2022 Ph.D. in Medical Biology, University of Tromsø, The Arctic University of Norway, supervisor Peter McCourt, co-supervisor Karen Sørensen, thesis: Novel screening methods for nanoscale changes in liver cell fenestrations elicited by pharmaceuticals
Research experience:
05.2022-present Researcher at Vascular Biology Research Group at University of Tromsø, The Arctic University of Norway, in EU EIC Pathfinder project DELIVERy
2018-2022 Early Stage Researcher at Vascular Biology Research Group at University of Tromsø, The Arctic University of Norway, ERS in the MSCA ITN project DeLIVER
08.2019-03.2020 Visiting research student at ANZAC Research Institute, University of Sydney, Australia and The ithree Institute, University of Technology Sydney (UTS), Australia
2015-2018 Intern at Laboratory of Experimental Pharmacology of Endothelium, Jagiellonian Centre for Experimental Therapeutics (JCET), Poland
2012-2018 Student Researcher at Centre for Nanometer-Scale Science and Advanced Materials (Nanosam), Jagiellonian University, Poland
Publication record:
As first/last/corresponding author:
- Szafranska K., Sorensen K., Lalor P., McCourt P. Sinusoidal cells and liver immunology (book chapter) in Sinusoidal Cells in Liver Diseases editor J. Gracia-Sancho , 2024 https://doi.org/10.1016/B978-0-323-95262-0.00003-6
- Szafranska K.*, Mao H.*, Kruse L., Holte C., Wolfson D., Ahluwalia B.S., Whitchurch C., Cole L., Lockwood G., Diekmann R., Le Couteur D., Cogger V., McCourt P. Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure, Scientific Reports 2023 https://doi.org/10.1038/s41598-023-40227-0
- Szafranska K., Neuman T., Baster Z., Rajfur Z., Szelest O., Holte C., Kubisiak A., Kus E., Wolfson D., Chlopicki S.,Ahluwalia B.S., Lekka M., Szymonski M., McCourt P., Zapotoczny B. From fixed-dried to wet-fixed to live–comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations, Nanophotonics 2022, https://doi.org/10.1515/nanoph-2021-0818
- Szafranska K., Holte C., Kruse L., Mao H., Øie C., Szymonski M., Zapotoczny B., McCourt P. Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study, Micron 2021, https://doi.org/10.1016/j.micron.2021.103121
- Szafranska K.*, Kruse L.*, Holte C.*, McCourt P., Zapotoczny B. The wHole story about fenestrations in LSEC,Frontiers in Physiology 2021, https://doi.org/10.3389/fphys.2021.735573
- Szafranska K.*, B. Zapotoczny B.*, Kus E., Chlopicki S., Szymonski M. Quantification of Fenestrations in Liver Sinusoidal Endothelial Cells by Atomic Force Microscopy, Micron 2017, https://doi.org/10.1016/j.micron.2017.06.005
As co-author:
- Holte C., Szafranska K., Kruse L., Simon-Santamaria J., Li R., Svistounov D., McCourt P. Highly oxidized albumin is mainly cleared by mouse liver sinusoidal endothelial cells via the receptors stabilin-1 and-2 Scientific Reports 2023 https://doi.org/10.1038/s41598-023-46462-9
- Engdahl A., Dutta S., Belle S., Schürstedt J., Szafranska K., McCourt P., Hellmann R., Huser T., Schüttpelz M. Dual-color single molecule localization microscopy on transparent polymer waveguide chips, bioRxiv 2023 (under review at ACS Nano) https://doi.org/10.1101/2022.11.29.518375
- Ortkrass H.,Wiebusch G., Linnenbrügger J., Schürstedt J. Szafranska K., McCourt P., Huser T. Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope, Optics Express 2023, https://doi.org/10.1364/OE.504292
- Ortkrass H., Schürstedt J., Wiebusch G., Szafranska K., McCourt P., Huser T. High-speed TIRF and 2D superresolution structured illumination microscopy with large field of view based on fiber optic components, Otpics Express 2023 (editors pick), https://doi.org/10.1364/OE.495353
- Shepherd E., Northall E., Papakyriacou P., Szafranska K., Sorensen K., Lalor P. Decellularization of the human liver to generate native extracellular matrix for use in automated functional assays with stellate cells in “Hepatic Stellate Cells: Methods and Protocols” Springer 2023
- Zapotoczny B., Szafranska K., Lekka M., Ahluwalia B.S., McCourt P. Tuning of Liver Sieve: The Interplay between Actin and Myosin Regulatory Light Chain Regulates Fenestration Size and Number in Murine Liver Sinusoidal Endothelial Cells International Journal of Molecular Sciences 2022, https://doi.org/10.3390/ijms23179850
- Van Os E., Cools L., Eysackers N., Szafranska K., Smout A., Verhulst S., Reynaert H., McCourt P., Mannaerts I., van Grunsven L. Modelling fatty liver disease with mouse liver-derived multicellular spheroids, Biomaterials 2022 https://doi.org/10.1016/j.biomaterials.2022.121817
- Butola A., Coucheron D., Szafranska K., Ahmad A., Mao H., Tinguely J-C., McCourt P., Senthilkumaran P., Mehta D.S., Agarwal K., Ahluwalia B.S. Quantitative phase imaging and on-chip nanoscopy for 3D imaging of liver sinusoidal endothelial cells, Digital Holography and Three-Dimensional Imaging, 2022 https://opg.optica.org/abstract.cfm?URI=DH-2022-W4A.2
- Butola A., Coucheron D., Szafranska K., Ahmad A., Mao H., Tinguely J-C., McCourt P., Senthilkumaran P., Mehta D.S., Agarwal K., Ahluwalia B.S. Multi-modal on-chip nanoscopy and quantitative phase image reveals the morphology of liver sinusoidal enodthelial cells, PNAS 2021, https://doi.org/ 10.1073/pnas.2115323118
- Kus E., Kaczara P., Czyzynska-Cichon I., Szafranska K., Zapotoczny B., Kij A., Sowinska A., Kotlinowski J., Mateuszuk L., Czarnowska E., Szymonski M., Chlopicki S. LSEC fenestrae are preserved despite proinflammatory phenotype of liver sinusoidal endothelial cells in mice on high fat diet, Frontiers in physiology 2019, https://doi.org/10.3389/fphys.2019.00006
- Zapotoczny B., Szafranska K., Kus E., Braet F., Wisse E., Chlopicki S., Szymonski M. Tracking fenestrae dynamics in live murine liver sinusoidal endothelial cells, Hepatology 2019, https://doi.org/10.1002/hep.30232
- Zapotoczny B., Szafranska K., Owczarczyk K., Kus E., Chlopicki S., Szymonski M. Atomic force microscopy reveals the dynamic morphology of fenestrations in live liver sinusoidal endothelial cells, Scientific Reports 2017, https://doi.org/10.1038/s41598-017-08555-0
- Zapotoczny B., Owczarczyk K., Szafranska K., Kus E., Chlopicki S., Szymonski M. Morphology and force probing of primary murine liver sinusoidal endothelial cells, Journal of Molecular Recognition 2017 https://doi.org/10.1002/jmr.2610
- Kochan K., Maslak E., Filipek A., Szafranska K., Chlopicki S., Baranska M. Label-free spectroscopic characterization of live liver sinusoidal endothelial cells (LSECs) isolated from the murine liver Analyst 2016, https://doi.org/10.1039/C6AN02063A