Daria Popova

Avhandlingen er tilgjengelig her! The doctoral thesis! Auditoriet er åpent for publikum, men disputasen vil også bli strømmet her. Opptak av disputasen vil være tilgjengelig i et døgn. The auditorium is open to the public. The defense will still be streamed here. A recording of the disputation will be available for 24 hours. Prøveforelesning over oppgitt emne holdes kl. 10.15, samme sted: “The current methods to select spermatozoa for cryopreservation and ICSI”. Prøveforelesningen vil også bli strømmet/the trail lecture will also be streamed.

Populærvitenskapelig sammendrag av avhandlingen:

Male infertility is a significant reproductive health concern. Assisted Reproductive Technologies (ART) have provided great hope for male infertility treatment, but the success rate is still relatively low.
The search of new semen parameters for male fertility evaluation and the exploration of the optimal method of sperm selection is one of the research priorities to improve ART outcomes.
The label-free Quantitative Phase Microscopy (QPM) coupled with image processing by Artificial Intelligence (AI) enabled us to detect tiny morphologic changes of spermatozoa under oxidative stress and recognize between healthy and stress-affected sperm cells. QPM-AI framework appears to be a promising tool in the automated classification and selection of sperm cells during ART procedures. Moreover, we set up the high-resolution imaging of living spermatozoa using label-based Structured Illumination Microscopy. This technique shows great promise for shedding new light on the detailed understanding of sperm cell functioning. In addition, we summarized the evidence from published literature regarding the association between semen quality and mitochondrial DNA copy numbers, which seems to be a relevant parameter in male fertility assessment.

Hovedveileder
Professor Purusotam Basnet, Institutt for klinisk medisin, Det helsevitenskapelige fakultet, UiT Norges arktiske universitet.

Biveiledere
Professor Ganesh Acharya, Institutt for klinisk medisin, Det helsevitenskapelige fakultet, UiT Norges arktiske universitet.

Professor Balpreet Singh Ahluwalia, Institutt for fysikk og teknologi, Fakultet for naturvitenskap og teknologi, UiT Norges arktiske universitet.

Bedømmelseskomité
Professor Juha Tapanainen, Department of Obstetrics and Gynecology University of Helsinki and Helsinki University Hospital - 1. opponent.

Professor emerita Tihana Žanić  Grubišić, Faculty of Pharmacy and Biochemistry, University of Zagreb- 2. opponent.

Professor Gøril Eide Flaten, Institutt for farmasi, Det helsevitenskapelige fakultet, UiT Norges arktiske universitet – leder av komité.

Disputasleder
Førsteamanuensis Vegard Brun, Institutt for klinisk medisin, Det helsevitenskapelige fakultet, UiT Norges arktiske universitet.