autumn 2019

BIO-3022 Biological membranes and their proteins - 5 ECTS

Sist endret: 20.09.2019

The course is provided by

Faculty of Biosciences, Fisheries and Economics

Location

Tromsø |

Application deadline

Concerns only admission to singular courses: Applicants from Nordic countries: 1 June for the autumn semester. Exchange students and Fulbright students: 15 April for the autumn semester.

Type of course

Master course for biology students - principally aimed at MSc-students specializing in Molecular Environmental Biology. Master course for chemistry students.

The course will be arranged with a maximum of 10 and minimum of 3 students.

Admission requirements

Local admission , application code 9371 - - Master`s level singular course.

Admission requires a Bachelor`s degree (180 ECTS) or equivalent qualification, with a major in biology or chemistry of minimum 80 ECTS.

The course will be arranged with a maximum of 10 and minimum of 3 students.

Course content

Membrane proteins are responsible for most communications between cells or cellular compartments. They ensure the passage of ions, metabolites or other molecules and permit signaling. They are involved in many fundamental processes (neurotransmission, bioenergetics, cell development and many others). Their mechanisms of action rely on minute atomic properties as well as on large conformational changes occurring within their structures. However, in contrast to soluble proteins, the function of a membrane protein is also strongly dependent on the properties of the lipidic membrane in which the protein is embedded. Because of the essential roles of membrane proteins in most fundamental biological processes, they constitute major drug targets, and it is estimated that nearly 60% of the currently approved drugs target membrane proteins. The course is an introduction to biological membranes. Students will learn about the composition of these membranes, lipids and proteins, the behavior of lipids and their phase diagrams, the role of proteins and the different types of functions they achieve. The course will give a complete overview from genes (and how to recognize genes encoding membrane proteins) to the synthesis and insertion pathways of membrane proteins to their target membrane. The courses will be of general interest to biology or chemistry students, with a particular focus on membrane processes deciphered at the molecular level. The course will also bring insights into biophysics of proteins. The course is held for 2 weeks and contains 14 lectures and 5 days in the lab.

Lectures are grouped in 4 parts: 1) Lipids and Membranes (4 lectures) Lipids and Biomembranes: definition of different classes of lipids, different phases formed by lipids in water Properties of biological membranes: lipid composition and membrane proteins Examples of different membranes in eukaryotic cells (specific lipids, ratio between lipids and proteins) Lipids of organisms living under extreme conditions (low temperature, high pressure) how to maintain the fluid phase 2) Membrane proteins in vivo and main biological functions (5 lectures) Finding membrane protein encoding genes within genomes, TM predictions, Topologies of membrane proteins Synthesis and transport to membranes Main functional classes: Transporters, Receptors, Ion channels, Enzymes Protein structures and their implications in function and dynamics 3) Methods for studying membranes and membrane proteins: biochemistry, structural biology and biophysics (4 lectures) Biochemistry of membrane proteins: Solubilization, purification, crystallization Properties and role of detergents and surfactants Liposomes and functional assays Biophysical methods to study membranes Single molecule fluorescence to understand membrane proteins in cells (dynamics, segregation, oligomerization) 4) Membrane proteins and drug design (1 lecture) Which targets? Identification of new hits Strategy for rational drug design

Lab exercises will be based on AcrB a multidrug transporter from E. coli, and will comprise: 1) Membrane preparation starting from cells overexpressing AcrB 2) Solubilization of membranes, purification of AcrB with affinity columns, test of a few detergents 3) Characterization of the purified protein,SDS-Page, Western Blot, Thermal stability 4) Crystallization of AcrB     5) Reconstitution into liposomes, testing protein incorporation by sucrose gradient and identification of the fractions

Objectives of the course

The student will get

  • extensive knowledge on lipids constituting biological membranes and the different phases they are able to form
  • profound insight into membrane proteins: how to recognize membrane proteins from genomes and what are the expected topologies, how they are synthesized in cells, which are the main functions fulfilled by membrane proteins
  • knowledge of the methods that are adapted to study membranes and membrane proteins, from biochemistry, biophysics and structural biology
  • insight into the process of drug design and knows which classes of membrane proteins constitute interesting targets
  • experience with the use of detergents for the solubilization of biological membranes and their purification, as well as insight into the crystallization process

The student

  • will acquired basic knowledge on the chemical nature and physical properties of lipid and detergent molecules
  • can outline the main topologies encountered in structures of membrane proteins
  • will know the main steps involved in membrane protein synthesis from the ribosome to protein insertion into the target membrane
  • will acquire an overall overview of the main technics to study isolated membrane proteins or biological membranes

The student

  • Understands the relevance of membrane proteins in fundamental biological pathways and their implications in pathologies
  • Understands the potential exploitation of membrane proteins for medical or biotechnical applications
  • Knows how to read a scientific text (report, or publication) related to membrane studies

Language of instruction

English

Teaching methods

The course is a 2-weeks intensive course with 10 working days (from 8h15 to 16h) comprising 14 lectures and lab exercises, plus home work. In total, 14h of lectures and 65h of lab work.

Assessment

Four hours written exam approximately 2 weeks after the course.
The assessment of the final exam will be pass or fail.

Coursework requirements: Admission to the examination requires that a minimum of 80% of the theoretical and practical parts of the course has been completed and that lab work has been reported satisfactorily.

Re-sit exam:
There will be a re-sit examination for students that did not pass the previous ordinary examination.

Date for examination

Written examination 11.10.2019

The date for the exam can be changed. The final date will be announced in the StudentWeb early in May and early in November.

Schedule



Kontakt
10628570_10156023205755596_3274667516272748229_n.jpg

Camilla Andreassen


Student Advisor
Telefon: +4777623206 camilla.andreassen@uit.no

Maren-Marie-Thode-Iversen-Bredde-180px-

Maren Marie Thode Iversen


Rådgiver
Telefon: +4777646610 maren.m.iversen@uit.no

Karsten-Fischer

Karsten Bruno Fischer


Professor
Telefon: +4777644114 karsten.fischer@uit.no