Marbio – an analytical platform for natural products

Experience in screening complex mixtures (i.e. natural product extracts and fractions) for biological activity in biochemical and cell-based assays
Identification of known and potentially unknown bioactive compounds (dereplication) using high-resolution tandem mass spectrometry
Mass guided isolation of bioactive natural products from extracts and organisms, purity determination.
Structural elucidation of complex natural products using high resolution mass spectrometry and spectroscopical tecniques such as NMR, IR and UV-Vis spectroscopy.
Isolation and cultivation of marine microorganisms (Bacteria and Fungi), genome mining
Focus on samples of marine origin such as extracts from invertebrates, marine fungi and bacteria.

Marbio prepares and screens fraction libraries from extracts provided by Marbank and provides technology and competence within the area of high-throughput screening and natural products drug discovery. The workflow in Marbio relies heavily on the automation of sample handling, preparation and analysis, and this is true for all the three workstations; purification, bioactivity screening and identification.

The tight integration of robust screening assays, early identification of known compounds and bioassay-guided purification of novel bioactive compounds is of great advantage.

Infrastructure

Autopurification HPLC (isolation of natural products)
High-resolution MS (dereplication)
Biomek NXp liquid handler
Envision Alphascreen multimode detector
High-resolution MS-MS and ion mobility (dereplication)
Tecan Spark multimode microplate reader
Q-Pix colony picker