Selected publications
FNAIT and platelet immunology
Adding contrast to shed some light - A case of acute thrombocytopenia due to iohexol-dependent antibodies to glycoprotein IIb/IIIa. Lægreid IJ, Hald EM, Arsenovic MG, Ernstsen SL, Asplund Högelin K, Mørtberg TV, Ahlen MT.
J Thromb Haemost. 2025; S1538-7836(25)00607-5. https://doi.org/10.1016/j.jtha.2025.09.016 Online ahead of print.
Improving prediction of fetal/neonatal intracranial hemorrhage in HPA-1a alloimmunized pregnancies to guide antenatal management: An observational cohort study. Ernstsen SL, Ahlen MT, Bertelsen EL, Kjeldsen-Kragh J, Husebekk A, Tiller H. Acta Obstet Gynecol Scand. 2025;104(10):1859-1868. https://doi.org/10.1111/aogs.70031
Rising to the challenge: an international Delphi consensus study on fetal and neonatal alloimmune thrombocytopenia. Pothof R, van den Akker-van Marle EM, de Vos TW, Tiller H, Bussel JB, Ahlén MT, Curtis BR, Lopriore E, Verweij EJTJ, de Haas M: FNAIT modified Delphi expert group. Lancet Haematol. 2025;12(4):e304-e311. https://doi.org/10.1016/S2352-3026(25)00029-8
Placenta-associated biomarkers and pregnancy outcome in HPA-1a alloimmunization: A prospective cohort study. Nedberg NH, Nystad M, Ahlen MT, Bertelsen EL, Guz K, Uhrynowska M, Dębska M, Gierszon A, Orzińska A, Husebekk A, Brojer E, Staff AC, Tiller H. Placenta. 2024;158:185-191. https://doi.org/10.1016/j.placenta.2024.10.014
What's with the boys? Lower birth weight in boys from HPA-1a alloimmunized pregnancies - New insights from a large prospective screening study in Poland. Coucheron T, Uhrynowska M, Guz K, Orzińska A, Debska M, Gierszon A, Ahlen MT, Bertelsen EL, Berge G, Husebekk A, Brojer E, Tiller H. J Reprod Immunol. 2023;160:104168. https://doi.org/10.1016/j.jri.2023.104168
Antenatal intravenous immunoglobulins in pregnancies at risk of fetal and neonatal alloimmune thrombocytopenia: comparison of neonatal outcome in treated and nontreated pregnancies. Ernstsen SL, Ahlen MT, Johansen T, Bertelsen EL, Kjeldsen-Kragh J, Tiller H. Am J Obstet Gynecol. 2022;227(3):506.e12. https://doi.org/10.1016/j.ajog.2022.04.044
Prevention of Fetal/Neonatal Alloimmune Thrombocytopenia in Mice: Biochemical and Cell Biological Characterization of Isoforms of a Human Monoclonal Antibody. Mortberg TV, Zhi H, Vidarsson G, Foss S, Lissenberg-Thunnissen S, Wuhrer M, Michaelsen TE, Skogen B, Stuge TB, Andersen JT, Newman PJ, Ahlen MT. Immunohorizons. 2022;6:90-103 https://doi.org/10.4049/immunohorizons.2100097
Acute drug-induced immune thrombocytopenia - A work of articaine. Laegreid IJ, Olsen MI, Harr JI, Grønli RH, Mørtberg TV, Ernstsen SL, Ahlen MT. Transfusion. 2022;62(5):1142-1147. https://doi.org/10.1111/trf.16858
Thrombosis and thrombocytopenia after HPV vaccination. Johansen S, Laegreid IJ, Ernstsen SL, Azrakhsh NA, Kittang AO, Lindås R, Gjertsen BT, Vetti N, Mørtberg TV, Sørvoll IH, Holme PA, Ahlen MT, Reikvam H. J Thromb Haemost. 2022;20(3):700-704. https://doi.org/10.1111/jth.15604
An observational study to identify the prevalence of thrombocytopenia and anti-PF4/polyanion antibodies in Norwegian health care workers after COVID-19 vaccination. Sørvoll IH, Horvei KD, Ernstsen SL, Laegreid IJ, Lund S, Grønli RH, Olsen MK, Jacobsen HK, Eriksson A, Halstensen AM, Tjønnfjord E, Ghanima W, Ahlen MT. J Thromb Haemost. 2021;19(7):1813-1818. https://doi.org/10.1111/jth.15352
Platelet alloimmunization is associated with low grade chronic histiocytic intervillositis - A new link to a rare placental lesion? Nedberg NH, Turowski G, Guz 3, Przytuła E, Uhrynowska M, Roald B, Husebekk A, Sitras V, Nystad M, Dębska M, Brojer E, Tiller H. Placenta. 2021;1:112:89-96. https://doi.org/10.1016/j.placenta.2021.07.291
Preclinical evaluation of immunotherapeutic regimens for fetal/neonatal alloimmune thrombocytopenia. Zhi H, Ahlen MT, Skogen B, Newman DK, Newman PJ. Blood Adv. 2021;5(18):3552-3562. https://doi.org/10.1182/bloodadvances.2021004371.
Thrombosis and Thrombocytopenia after ChAdOx1 nCoV-19 Vaccination. Schultz NH, Sørvoll IH, Michelsen AE, Munthe LA, Lund-Johansen F, Ahlen MT, Wiedmann M, Aamodt AH, Skattør TH, Tjønnfjord GE, Holme PA. N Engl J Med. 2021;384(22):2124-2130. https://doi.org/10.1056/NEJMoa2104882
The prevalence of HPA-1a alloimmunization and the potential risk of FNAIT depend on both the DRB3*01:01 allele and associated DR-DQ haplotypes. Ahlen MT, Heide G, Husebekk A, Skogen B, Kjeldsen-Kragh J, Stuge TB. Scand J Immunol. 2020;92(1):e12890.
https://doi.org/10.1111/sji.12890
T cell responses to Human Platelet Antigen-1a involve a unique form of indirect allorecognition. Ahlen MT, Husebekk A, Killie IL, Skogen B, Stuge TB. JCI Insight. 2016;1(14):e86558 http://doi.org/10.1172/jci.insight.86558
Characterization of a Human Platelet Antigen-1a-Specific Monoclonal Antibody Derived from a B Cell from a Woman Alloimmunized in Pregnancy. Eksteen M, Tiller H, Averina M, Heide G, Kjaer M, Ghevaert C, Michaelsen TE, Ihle O, Husebekk A, Skogen B, Stuge TB. J Immunol. 2015;194: 5751-5760. https://doi.org/10.4049/jimmunol.1401599
Toward a prophylaxis against fetal and neonatal alloimmune thrombocytopenia: induction of antibody-mediated immune suppression and prevention of severe clinical complications in a murine model. Tiller H, Killie MK, Chen P, Eksteen M, Husebekk A, Skogen B, Kjeldsen-Kragh J, Ni H. Transfusion. 2012;52:1446-1457. https://doi.org/10.1111/j.1537-2995.2011.03480.x
T-cell responses associated with neonatal alloimmune thrombocytopenia: isolation of HPA-1a-specific, HLA-DRB3*0101-restricted CD4+ T cells. Ahlen MT, Husebekk A, Killie MK, Skogen B and Stuge TB. Blood. 2009;16;113(16):3838-3844. https://doi.org/10.1182/blood-2008-09-178475
A screening and intervention program aimed to reduce mortality and serious morbidity associated with severe neonatal alloimmune thrombocytopenia. Kjeldsen-Kragh J, Killie MK, Tomter G, Golebiowska E, Randen I, Hauge R, Aune B, Oian P, Dahl LB, Pirhonen J, Lindeman R, Husby H, Haugen G, Gronn M, Skogen B, Husebekk A. Blood. 2007;1;110(3):833-839. https://doi.org/10.1182/blood-2006-08-040121
Immunothrombosis
Extracellular vesicles from activated platelets possess a phospholipid-rich biomolecular profile and enhance prothrombinase activity. Guerreiro EM, Kruglik SG, Swamy S, Latysheva N, Østerud B, Guigner JM, Sureau F, Bonneau S, Kuzmin AN, Prasad PN, Hansen JB, Hellesø OG, Snir O. J Thromb Haemost. 2024;22:S1538-7836(24)00041-2. https://doi.org/10.1016/j.jtha.2024.01.004
Sustained and intermittent hypoxia differentially modulate primary monocyte immunothrombotic responses to IL-1β stimulation. Wahlund CJE, Çaglayan S, Czarnewski P, Hansen JB, Snir O. Front Immunol. 2023;11;14:1240597. https://doi.org/10.3389/fimmu.2023.1240597
Plasma levels of platelet-derived microvesicles are associated with risk of future venous thromboembolism. Snir O, Wilsgård L, Latysheva N, Wahlund CJE, Braekkan SK, Hindberg K, Hansen JB. J Thromb Haemost. 2022;20(4):899-908. https://doi.org/10.1111/jth.15638
A rapid, sensitive, and specific assay to measure TF activity based on chromogenic determination of thrombin generation. Østerud B, Latysheva N, Schoergenhofer C, Jilma B, Hansen JB, Snir O. J Thromb Haemost. 2022;20(4):866-876. https://doi.org/10.1111/jth.15606.
Virus Immunity Pathology (VIP)
Hirsch, H. H. (2023). Polyomaviruses. In L. Goldman & K. A. Cooney (Eds.), Goldman-Cecil Medicine (27th ed., Vol. 2, pp. 2227-2233). Elsevier. https://www.asia.elsevierhealth.com/9780323930383.html
DeCaprio, J. A., Imperiale, M. J., & Hirsch, H. H. (2021). Polyomaviridae. In P. M. Howley & D. M. Knipe (Eds.), Fields Virology (7 ed., Vol. 2: DNA Viruses, pp. 1-44). Lippincott Williams & Wilkins. https://shop.lww.com/Fields-Virology--DNA-Viruses/p/9781975112578
Leuzinger, K., & Hirsch, H. H. (2023). Human polyomaviruses. In K. C. Carroll & M. A. Pfaller (Eds.), Manual of Clinical Microbiology (13th ed., pp. 2093-2130). ASM Press. https://www.clinmicronow.org/doi/10.1128/9781683670438.MCM.ch109
Kotton, C. N., Kamar, N., Wojciechowski, D., Eder, M., Hopfer, H., Randhawa, P., Sester, M., Comoli, P., Tedesco Silva, H., Knoll, G., Brennan, D. C., Trofe-Clark, J., Pape, L., Axelrod, D., Kiberd, B., Wong, G., Hirsch, H. H., & Transplantation Society International BK Polyomavirus Consensus Group. (2024). The Second International Consensus Guidelines on the Management of BK Polyomavirus in Kidney Transplantation. Transplantation, 108(9), 1834-1866. https://doi.org/10.1097/TP.0000000000004976
Kaur, A., Wilhelm, M., Wilk, S., & Hirsch, H. H. (2019). BK polyomavirus-specific antibody and T-cell responses in kidney transplantation: update. Curr Opin Infect Dis, 32(6), 575-583. https://doi.org/10.1097/qco.0000000000000602
Cesaro, S., Dalianis, T., Hanssen Rinaldo, C., Koskenvuo, M., Pegoraro, A., Einsele, H., Cordonnier, C., Hirsch, H. H., & ECIL-6 Group. (2018). ECIL guidelines for the prevention, diagnosis and treatment of BK polyomavirus-associated haemorrhagic cystitis in haematopoietic stem cell transplant recipients. J Antimicrob Chemother, 73(1), 12-21. https://doi.org/10.1093/jac/dkx324
Hirsch, H. H., Kaufmann, G., Sendi, P., & Battegay, M. (2004). Immune reconstitution in HIV infected patients. Clin Infect Dis, 38(8), 1159-1166. https://doi.org/10.1086/383034
Durairaj, J., Follonier, O. M., Leuzinger, K., Alexander, L. T., Wilhelm, M., Pereira, J., Hillenbrand, C. A., Weissbach, F. H., Schwede, T., & Hirsch, H. H. (2024). Structural implications of BK polyomavirus sequence variations in the major viral capsid protein Vp1 and large T-antigen: a computational study. mSphere, 9(4), e0079923. https://doi.org/10.1128/msphere.00799-23
Leuzinger, K., Kaur, A., Wilhelm, M., Frank, K., Hillenbrand, C. A., Weissbach, F. H., & Hirsch, H. H. (2023). Molecular Characterization of BK Polyomavirus Replication in Allogeneic Hematopoietic Cell Transplantation. J Infect Dis, 227(7), 888-900. https://doi.org/10.1093/infdis/jiac450
Weissbach, F. H., Follonier, O. M., Schmid, S., Leuzinger, K., Schmid, M., & Hirsch, H. H. (2024). Single-cell RNA-sequencing of BK polyomavirus replication in primary human renal proximal tubular epithelial cells identifies specific transcriptome signatures and a novel mitochondrial stress pattern. J Virol, 98(12), e0138224. https://doi.org/10.1128/jvi.01382-24
Wilhelm, M., Kaur, A., Geng, A., Wernli, M., & Hirsch, H. H. (2025). Donor Variability and PD-1 Expression Limit BK Polyomavirus-specific T-cell Function and Therapy. Transplantation, 109(9), 1526-1539. https://doi.org/10.1097/TP.0000000000005399
Hillenbrand, C. A., Akbari Bani, D., Follonier, O., Kaur, A., Weissbach, F. H., Wernli, M., Wilhelm, M., Leuzinger, K., Binet, I., Bochud, P. Y., Golshayan, D., Hirzel, C., Manuel, O., Mueller, N. J., Schaub, S., Schachtner, T., Van Delden, C., Hirsch, H. H., & Swiss Transplant Cohort Study. (2025). BK polyomavirus serotype-specific antibody responses in blood donors and kidney transplant recipients with and without new-onset BK polyomavirus-DNAemia: A Swiss Transplant Cohort Study. Am J Transplant, 25(5), 985-1001. https://doi.org/10.1016/j.ajt.2024.11.019
Manzetti, J., Weissbach, F. H., Graf, F. E., Unterstab, G., Wernli, M., Hopfer, H., Drachenberg, C. B., Rinaldo, C. H., & Hirsch, H. H. (2020). BK Polyomavirus Evades Innate Immune Sensing by Disrupting the Mitochondrial Network and Promotes Mitophagy. iScience, 23(7), 101257. https://doi.org/10.1016/j.isci.2020.101257
Meier, R. P. H., Muller, Y. D., Dietrich, P. Y., Tille, J. C., Nikolaev, S., Sartori, A., Labidi-Galy, I., Ernandez, T., Kaur, A., Hirsch, H. H., McKee, T. A., Toso, C., Villard, J., & Berney, T. (2021). Immunologic Clearance of a BK Virus-associated Metastatic Renal Allograft Carcinoma. Transplantation, 105(2), 423-429. https://doi.org/10.1097/tp.0000000000003193
Müller, D. C., Rämö, M., Naegele, K., Ribi, S., Wetterauer, C., Perrina, V., Quagliata, L., Vlajnic, T., Ruiz, C., Balitzki, B., Grobholz, R., Gosert, R., Ajuh, E. T., Hirsch, H. H., Bubendorf, L., & Rentsch, C. A. (2018). Donor-derived, metastatic urothelial cancer after kidney transplantation associated with a potentially oncogenic BK polyomavirus. J Pathol, 244(3), 265-270. https://doi.org/10.1002/path.5012
