Procoagulant activity of extracellular vesicles

We have recently shown that elevated levels of extracellular vesicles (EVs) derived from activated platelets are associated with risk of future VTE. High levels of platelet-EVs are also found in the acute phase of the disease. In this project we will investigate the procoagulant function of platelet-derived EVs. To enable exclusive detection of EVs from activated platelets, we have defined a highly specific panel of proteins and phospholipids that are displayed by EVs derived from activated platelets. In addition, we have developed and validated two high throughput methodologies, beyond the state-of-the-art, to detect platelet-EVs: (i) Bead-based flow cytometry to target specific markers that are displayed by platelet-EVs and (ii) Raman tweezers microspectroscopy (RTM) to monitors the unique biochemical profile at the single-EV levels. 

Read more about the trombosis and trombosis-related projects at the Trombosis Research Group webpage@UiT 

Members:

Collaborators:

Olav Gaute Hellesø and Balpreet Singh Ahluwalia (Department of Physics and Technology, UiT)

Publications: 

Plasma levels of platelet-derived microvesicles are associated with risk of future venous thromboembolism

A rapid, sensitive, and specific assay to measure TF activity based on chromogenic determination of thrombin generation

Plasma procoagulant phospholipid clotting time and venous thromboembolism risk

Financial/grant information:

Nasjonalforeningen for folkehelsen