DC8
Dynamic interplay and crosstalk between hub molecules (acetyl-CoA, SAM) and their PTMs (histone acetylation & methylation)
Background: It is well known that protein modification signatures change in response to metabolic shifts. However, it is largely unknown how the cellular availability and dynamics of hub molecules are reflected in PTMs and, conversely, how changes in the persistence of specific PTMs affect the activities of specific metabolic pathways for hub molecule production itself. In DC6, innovative technologies are being developed to study the interplay between the availability and turnover of acetyl-CoA and SAM and the dynamics and crosstalk of histone acetylation and methylation.
Objectives: (1) LC-MS-based quantification of Acetyl-CoA and SAM production and their biosynthetic intermediates (2) Stable-isotope-based quantification of PTM (acetylation, methylation) dynamics by high resolution LC-MS (3) Quantification of PTM dynamics and cross-talk upon hub molecule modulation