DC1

Background: Metabolic conversions and signaling functions of different hub molecules are interconnected. Previous work has also demonstrated that the level of one hub molecule can be limited by the deficiency of another one owing to its cofactor role. However, while these interdependencies are critical for cell homeostasis, they have not been systematically studied. Objectives: (1) Establish analytical procedures to determine concentrations and turnover rates of NAD, CoA, FAD, SAM and ATP and their biosynthetic intermediates (2) Set up suitable cell model systems with modulated HubMOL concentrations and turnovers (3) Establish interdependencies of hub molecule dynamics and exploit them to re-adjust imbalances.