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Høst 2024
BIO-3018 Environmental Molecular Genetics - 20 stp
The course is administrated by
Institutt for arktisk og marin biologi
Type of course
Master course for biology students - principally aimed at MSc-students specializing in Molecular Environmental Biology.
The course is not available as a singular course.
The number of participants is limited to 12 (including students from BIO-8018).
Course overlap
BIO-8018 Environmental Molecular Genetics 20 ects
Course contents
The aim of this course is to give a thorough introduction to molecular genetic methods and bioinformatics used in modern biological research. The course will give a basis for advanced studies in different areas of biology and include practical exercises followed by computer analyses of own data sets. The students will isolate DNA and RNA from sub-Arctic samples, and prepare samples for incubation, GC analyses, sequencing and bioinformatics in the first part of the course. The second part includes the study on the epigenetic regulation of gene expression in plants. The laboratory and computer work is running for four weeks and the results are discussed in plenum. The students have to write individual laboratory reports. The lectures will be closely connected to the practical problems to be solved in the laboratory. Selected articles from the scientific curriculum are topics for seminar presented by the students.
Objective of the course
Knowledge:
- Theory of microbial ecology and epigenetics
- Theory of gene and gene expression analyses
- Critical consideration of scientific literature within the topics for the course How to write a laboratory report
- Critical assessment of own results
Skills:
- Soil incubations and gas analyses
- Isolation of DNA and RNA from environmental samples
- Preparation of samples for sequencing
- Bioinformatics - analysis of sequencing results, sequence assemblies
- Gene expression (qPCR), methylation status analysis
- Present objectives, methods and results.
- Oral and written discussion of results
General competence:
- Theory of environmental genetics
- Experimental design
- Molecular laboratory work
- Evaluation of methods
- Discussion of results
- Presentation of results
Language of instruction
The language of instruction is English and all of the syllabus material is in English. Examination questions will be given in English, but may be answered either in English or a Scandinavian language.
Teaching methods
Lectures 12 hours, laboratory 60 hours, seminars 16 hours.