Stable isotope labelling approaches

The development of high resolution mass spectrometry technology now enables the measurement of dynamic changes in metabolites and protein modifications based on stable isotope labelling approaches. To make full use of the potential of this new technology, we need new data analysis pipelines that among others include a step to correct for natural occuring isotopes. The resulting time course data then need to be further analysed to unravel the underlying dynamics of the system.

Related publications

  1. Alienke van Pijkeren, Jörn Dietze, .... Ines Heiland, Rainer Bischoff, Marcel Kwiatkowski Combined Metabolic and Chemical (CoMetChem) labeling using stable isotopes–A strategy to reveal site-specific histone acetylation and deacetylation rates by LC-MS Analytical Chemistry  2021 93, 38, 12872–12880
  2. Dietze, J, van Pijkeren, A Ziegler, M, Kwiatkowski, M, Heiland, I Natural isotope correction improves analysis of protein modification dynamics Analytical and Bioanalytical Chemistry 2021413 (30), 7333-7340

Related data analysis pipeline

PICor is an open source toolbox for stable isotope correction of metabolites and peptids including protein modifications


Ines Heiland (Principal investigator)
Jørn Dietze