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Ann Mari Holsæter

Førsteamanuensis
Institutt for farmasi

Stillingsbeskrivelse:

Førsteamanuensis tilknyttet forskningsgruppen "Drug Transport and Delivery Research Group".

Kursansvarlig for emnet:

Underviser også i emnene:

  • FAR-2402; Legemiddelformulering og Biofarmasi
  • FAR-3911; Masteroppgave i farmasi (veileder)
  • FAR-3043: Advanced drug delivery systems in nanomedicine
  • FAR-8043; Nanomedicine and Nanotechnology



Forskningsinteresser

Mine forskningsinteresser samsvarer med forskningsinteressene til gruppa "Drug transport and delivery".

og angår formulering og optimalisering av legemiddelformuleringer for topical- (sårbehandling) og systemisk- (injeksjonsmedisin) administrasjo mtp:

  • Holdbarhet/stabilitet
  • Brukervennlighet
  • Effekt
  • Bivirkningsprofil
  • Fremstillingsmetode

Karakterisering av legemiddelformuleringens fysikalskjemiske-, biofarmasøytiske- og kliniske/terapeutiske egenskaper skjer vha a in vitro, ex vivo og in vivo testing.

 

Forskningsprosjekter

"Liposomes-in-gels delivery systems: From bench to product development"

Formålet med dette prosjektet, som er et Ph.D. prosjekt, er å utvikle unik(e) legemiddelformuleringer for topikal anvendelse med utgangspunkt i liposom- og hydrogelteknologi.

"Utvikling av nye sårprodukter med SBG som aktiv ingrediens"

Ph.D. nærings/Doktorgradsprosjekt i samarbeid med Biotec BetaGlucans AS

 

Biografiske data

Cand.Pharm fra UiT 1999, med hovedfag i Radiofarmasi (IsoPharma, Kjeller). Hovedfagsavhandlingen hadde tittelen "Utvikling av et preparasjonssett for merking av antisens oligodeoksynukleotider med Technetium-99m"

Dr. Scient fra UiT 2004, Tittel på avhandlingen var "Parenteral Liposome -and Cyclodextrine Formulation of Camptothecin"  

Etter doktorgraden har jeg jobbet som post.doc ved UiT, Insitutt for Farmasi (2004-2006), vært ansatt i ProBio (nå Ayanda) som kvalitetsansvarlig (2006-2008) og jobbet i ulike roller som kvalitetsansvarlig/laboratorieansvarlig og QP i Biotec Pharmacon (2008-2011).

Ansatt i min nåværende stilling som førsteamanuensis siden januar 2011. 

Verv

Varamedlem i "ledergruppen ved Institutt for farmasi" (fra november 2013)

Varemedlem for Institutt for farmasi i "brukerråd for AKM" (fra Oktober 2018)

 

 

Arbeider i CRIStin


  • Ternullo, Selenia; Basnet, Purusotam; Holsæter, Ann Mari; Flaten, Gøril Eide; de Weerd, Louis; Skalko-Basnet, Natasa. Deformable liposomes for skin therapy with human epidermal growth factor: The effect of liposomal surface charge. European Journal of Pharmaceutical Sciences 2018; Volum 125. ISSN 0928-0987.s 163 - 171.s doi: 10.1016/j.ejps.2018.10.005.

  • Grip, Jostein; Engstad, Rolf Einar; Skjæveland, Ingrid; Skalko-Basnet, Natasa; Isaksson, Johan; Basnet, Purusotam; Holsæter, Ann Mari. Beta-glucan-loaded nanofiber dressing improves wound healing in diabetic mice. European Journal of Pharmaceutical Sciences 2018; Volum 121. ISSN 0928-0987.s 269 - 280.s doi: 10.1016/j.ejps.2018.05.031.

  • Ternullo, Selenia; de Weerd, Louis; Flaten, Gøril Eide; Holsæter, Ann Mari; Skalko-Basnet, Natasa. The isolated perfused human skin flap model: A missing link in skin penetration studies?. European Journal of Pharmaceutical Sciences 2017; Volum 96. ISSN 0928-0987.s 334 - 341.s doi: 10.1016/j.ejps.2016.10.003.

  • Massing, Ulrich; Ingebrigtsen, Sveinung Gaarden; Skalko-Basnet, Natasa; Holsæter, Ann Mari. Dual Centrifugation - A Novel “in-vial” Liposome Processing Technique. (fulltekst) INTECH 2017 ISBN 978-953-51-3580-7.s 3 - 28.s doi: 10.5772/intechopen.68523.

  • Øverbye, Anders; Holsæter, Ann Mari; Fusser, Markus; Skalko-Basnet, Natasa; Iversen, Tore Geir; Torgersen, Maria Lyngaas; Sønstevold, Tonje; Engebråten, Olav; Flatmark, Kjersti; Mælandsmo, Gunhild; Skotland, Tore; Sandvig, Kirsten. Ceramide-containing liposomes with doxorubicin: time and cell-dependent effect of C6 and C12 ceramide. OncoTarget 2017; Volum 8 (44). ISSN 1949-2553.s 76921 - 76934.s doi: 10.18632/oncotarget.20217.

  • Ternullo, Selenia; de Weerd, Louis; Holsæter, Ann Mari; Flaten, Gøril Eide; Skalko-Basnet, Natasa. Going skin deep: a direct comparison of penetration potential of lipid-based nanovesicles on the isolated perfused human skin flap model. European journal of pharmaceutics and biopharmaceutics 2017; Volum 121. ISSN 0939-6411.s 14 - 23.s doi: 10.1016/j.ejpb.2017.09.006.

  • Grip, Jostein; Engstad, Rolf Einar; Skjærveland, Ingrid; Skalko-Basnet, Natasa; Holsæter, Ann Mari. Sprayable Carbopol hydrogel with soluble beta-1,3/1,6-glucan as an active ingredient for wound healing – Development and in-vivo evaluation. European Journal of Pharmaceutical Sciences 2017; Volum 107. ISSN 0928-0987.s 24 - 31.s doi: 10.1016/j.ejps.2017.06.029.

  • Ingebrigtsen, Sveinung Gaarden; Didriksen, Alena; Johannessen, Mona; Skalko-Basnet, Natasa; Holsæter, Ann Mari. Old drug, new wrapping – A possible comeback for chloramphenicol?. International Journal of Pharmaceutics 2017; Volum 526 (1-2). ISSN 0378-5173.s 538 - 546.s doi: 10.1016/j.ijpharm.2017.05.025.

  • Ingebrigtsen, Sveinung Gaarden; Skalko-Basnet, Natasa; Cavalcanti Jacobsen, Cristiane de Albuquerque; Holsæter, Ann Mari. Successful co-encapsulation of benzoyl peroxide and chloramphenicol in liposomes by a novel manufacturing method - dual asymmetric centrifugation. (fulltekst) European Journal of Pharmaceutical Sciences 2017; Volum 97. ISSN 0928-0987.s 192 - 199.s doi: 10.1016/j.ejps.2016.11.017.

  • Ingebrigtsen, Sveinung Gaarden; Skalko-Basnet, Natasa; Holsæter, Ann Mari. Development and optimization of a new processing approach for manufacturing topical liposomes-in-hydrogel drug formulations by dual asymmetric centrifugation. Drug Development and Industrial Pharmacy 2016; Volum 42 (9). ISSN 0363-9045.s 1375 - 1383.s doi: 10.3109/03639045.2015.1135940.

  • Vanić, Željka; Holsæter, Ann Mari; Skalko-Basnet, Natasa. (Phospho)lipid-based nanosystems for skin administration. Current pharmaceutical design 2015; Volum 21 (29). ISSN 1381-6128.s 4174 - 4192.s doi: 10.2174/1381612821666150901095838.

  • Holsæter, Ann Mari; Basnet, Purusotam; Skalko-Basnet, Natasa. Fra droger til moderne legemidler: Hvor viktig er formulering?. (fulltekst) Norsk Farmaceutisk Tidsskrift 2013; Volum 121 (7/8). ISSN 0029-1935.s 26 - 29.

  • Hupfeld, Stefan; Holsæter, Ann Mari; Skar, Merete L.; Frantzen, christer bakke; Brandl, Martin. Liposome size analysis by dynamic/static light scattering upon size exclusion-/field flow-fractionation. Journal of Nanoscience and Nanotechnology 2006; Volum 6. ISSN 1533-4880.s 3025 - 3031.

  • Holsæter, Ann Mari; Skar, Merete L.; Braaten, Åsmund; Brandl, Martin. Camptothecin-catalyzed phospholipid hydrolysis in liposomes. Internation Journal of Pharmaceutics 2005; Volum 288 (1). ISSN 0378-5173.s 73 - 80.

  • Sætern, Ann Mari; Flaten, Gøril Eide; Brandl, Martin. A method to determine the incorporetion capacity of camptothecin in liposomes. AAPS PharmSciTech 2004; Volum 5 (3). ISSN 1530-9932.

  • Sætern, Ann Mari; Brandl, Martin; Bakkelund, Wenche Helen; Sveinbjørnsson, Baldur. Cytotoxic effect of different camptothecin formulations on human colon carcinoma in vitro. Anti-Cancer Drugs 2004; Volum 15 (9). ISSN 0959-4973.s 899 - 906.

  • Sætern, Ann Mari; Nguyen, Nguyen B; Bauer-Brandl, Annette; Brandl, Martin. Effect of hydroxypropyl-beta-cyclodextrin-complexation and pH on solubility of camptothecin. Internation Journal of Pharmaceutics 2004; Volum 284. ISSN 0378-5173.s 61 - 68.

  • Sætern, Ann Mari; Flaten, Gøril Eide; Brandl, Martin. A Method to Determine the Incorporation Capacity of Camptothecin in Liposomes. AAPS PharmSciTech 2004; Volum 5 (3). ISSN 1530-9932.s 1 - 7.

  • Hjelstuen, Ole Kristian; Sætern, Ann Mari; Tønnesen, Hanne H; Bremer, P.O.; Verbruggen, A.M.. Development of a lyophilized kit formulation for labeling of DNA probes with 99mTc. Internation Journal of Pharmaceutics 1999; Volum 190 (2). ISSN 0378-5173.s 197 - 205.s doi: 10547460.

  • Holsæter, Ann Mari; Grip, Jostein; Skalko-Basnet, Natasa. Advanced wound dressing with beta-glucans: in vivo proof of nanofibers superiority. Northern pharma network and nordic POP kick off-meeting 2018 2018-01-29 - 2018-10-01 2018.

  • Grip, Jostein; Engstad, Rolf Einar; Skjæveland, Ingrid; Skalko-Basnet, Natasa; Holsæter, Ann Mari. Development of a novel wound healing spray: soluble beta-1,3/1,6-glucan as an active ingredient in Carbopol matrix in a db/db wound model. The European Advanced Materials Congress (EAMC) 2017-08-22 - 2017-08-24 2017.

  • Ternullo, Selenia; de Weerd, Louis; Holsæter, Ann Mari; Flaten, Gøril Eide; Skalko-Basnet, Natasa. A Nano-Approach to Improve Local Skin Therapy: Isolated Perfused Human Skin Flap as a Tool in Optimization. 2017 AAPS Annual Meeting and Exposition 2017-11-12 - 2017-11-15 2017.

  • Holsæter, Ann Mari; Ingebrigtsen, Sveinung Gaarden; Skalko-Basnet, Natasa. Dermal Application of Chloramphenicol - The Effect of Liposomes and Chitosan Hydrogel Formulations on ex vivo Permeation and Antimicrobial Activity. AAPS Annual meeting and exposition 2017 2017-11-12 - 2017-11-15 2017.

  • Holsæter, Ann Mari. New activities related to liposomes. NANOCAN meeting 2017-01-26 - 2017-02-27 2017.

  • Holsæter, Ann Mari. Preparation, characterization and in vitro testing of liposomes. NANOCAN meeting 2016-01-07 - 2016-01-08 2016.

  • Ternullo, Selenia; de Weerd, Louis; Flaten, Gøril Eide; Holsæter, Ann Mari; Skalko-Basnet, Natasa. Isolated perfused human skin flap model: a novel tool to evaluate drug penetration through the skin II. The 8th National PhD Conference in Medical Imaging 2016-11-21 - 2016-11-22 2016.

  • Ternullo, Selenia; de Weerd, Louis; Flaten, Gøril Eide; Holsæter, Ann Mari; Skalko-Basnet, Natasa. Isolated perfused human skin flap model: a novel tool to evaluate drug penetration through the skin. The 8th National PhD Conference in Medical Imaging 2016-11-21 - 2016-11-22 2016.

  • Øverbye, Anders; Holsæter, Ann Mari; Skalko-Basnet, Natasa; Tore-Geir, Iversen; Torgersen, Maria Lyngaas; Sønstevold, Tonje; Skotland, Tore; Sandvig, Kirsten. Biodegradable nanoparticles in cancer diagnosis and therapy (NANOCAN):Ceramide modulation of liposomal doxorubicin. Annual seminar for Center of Excellence – Center for Cancer Biomedicine 2016-09-21 - 2016-09-21 2016.

  • Ternullo, Selenia; de Weerd, Louis; Flaten, Gøril Eide; Holsæter, Ann Mari; Skalko-Basnet, Natasa. Isolated perfused human skin flap model: A novel tool to evaluate drug penetration through the skin. Skin Forum 2016 Annual Meeting 2016-06-21 - 2016-06-22 2016.

  • Holsæter, Ann Mari; Skalko-Basnet, Natasa. Liposomes as multi-functional delivery system in cancer therapy. NANOCANCER meeting 2015-01-08 - 2015-01-09 2015.

  • Holsæter, Ann Mari; Skalko-Basnet, Natasa. Multi-functional liposomes as delivery system in cancer therapy. Snakk med en forsker 2015-09-24 - 2015-09-24 2015.

  • Ingebrigtsen, Sveinung Gaarden; Laidemäe, Ivo; Saarinen, Jukka; Strachan, Clare J.; Holsæter, Ann Mari; Skalko-Basnet, Natasa; Heinämäki, Jyrki; Kogermann, Karin. Characterization of liposome-loaded nanofibers by coherent anti-Stokes Raman scattering microscopy and scanning white light interferometry. 6th BBBB - Conference on Pharmaceutical Sciences: Strategies to Improve the Quality and Performance of Modern Drug Delivery Systems 2015-09-10 - 2015-09-12 2015.

  • Ingebrigtsen, Sveinung Gaarden; Johansen, Kurt Jonny; Skalko-Basnet, Natasa; Holsæter, Ann Mari. A new preparation method for preparation of liposomes-in-hydrogels primed for treatment of skin diseases. Liposome Research Days 2014: Living Innovation 2014-08-04 - 2014-08-07 2014.

  • Ingebrigtsen, Sveinung Gaarden; Laidmäe, Ivo; Lust, Andres; Holsæter, Ann Mari; Skalko-Basnet, Natasa; Toom, Lauri; Paaver, Urve; Veski, Peep; Heinämäki, Jyrki; Kogermann, Karin. Preparation of liposomes and liposomes-in-nanofiber systems and their solid state analysis. Arstiteaduskonna konverentsile 2014-10-09 - 2014-10-10 2014.

  • Ingebrigtsen, Sveinung Gaarden; Laidmäe, Ivo; Lust, Andres; Holsæter, Ann Mari; Skalko-Basnet, Natasa; Toom, Lauri; Veski, Peep; Heinämäki, Jyrki; Kogermann, Karin. Preparation and solid state analysis of electrospun liposome-loaded nanofibers for topical wound healing. 8th PSSRC Annual Symposium 2014-09-16 - 2014-09-18 2014.

  • Holsæter, Ann Mari. Liposomes in skin delivery. EEA Norway Estonia meeting 2014-04-28 - 2014-04-30 2014.

  • Ingebrigtsen, Sveinung Gaarden; Skalko-Basnet, Natasa; Holsæter, Ann Mari. A novel one-step preparation method for liposomes using dual asymmetric centrifugation. 3rd Conference on Innovation in Drug Delivery: Advances in local drug delivery 2013-09-22 - 2013-09-25 2013.

  • Holsæter, Ann Mari; Skar, Merete L.; Brandl, Martin. Particle size analysis of nano-drug carriers using photon correlation spectroscopy and light obscuration particle counter. Nanomat Birkeland konferanse 2005-06-02 - 2005-06-03 2005.

  • Holsæter, Ann Mari; Brandl, Martin. Incorporation efficiency and in vitro cytotoxicity of camptothecin in liposomes. 15th International Symposium on Microencapsulation 2005-09-18 - 2005-09-21 2005.

  • Brandl, Martin; Skar, Merete L.; Frantzen, christer bakke; Ingebrigtsen, L; Holsæter, Ann Mari. Liposome size analysis by PCS, SEC and FFFF. 1st Meeting on Field Flow Fractionation 2005-10-06 - 2005-10-07 2005.

  • Holsæter, Ann Mari; Brandl, Martin. Liposomes as Nano-Carriers for Site-Specific Delivery of Anti-cancer Drugs. Nanomat Birkeland konferanse 2005-06-02 - 2005-06-03 2005.

  • Flaten, Gøril Eide; Sætern, Ann Mari; Brandl, Martin. Camptothecin-Lactone Incorporation in Liposomes - A Phase Solubility Study. International Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology 2004-03-27 - 2004-03-30 2004.

  • Sætern, Ann Mari. Parenteral Liposome- and Cyclodextrin Formulation of Camptothecin. 2004 ISBN 82-497-0234-4.

  • Sætern, Ann Mari; Sveinbjørnsson, Baldur; Flaten, Gøril Eide; Brandl, Martin. Camptothecin-in-liposomes: Incorporation capacity and in vitro cytotoxicity. The Midnight Sun Meeting on Drug Transport and Drug Delivery 2004-07-01 - 2004-07-03 2004.

  • Sætern, Ann Mari; Nguyen, Nguyen B; Brandl, Martin. Camptothecin Complexation with Hydroxypropyl-beta-Cyclodextrin - A phase-solubility study. Int. Meeting on Pharmaceutics, Biopharmaceutics, Pharmaceutical Technology 2004-03-27 - 2004-03-30 2004.

  • Sætern, Ann Mari. Camptothecin-in-liposomes: Incorporation capacity and in vitro cytotoxicity. The Midnight Sun Meeting on Drug transport and Drug delivery 2004-07-01 - 2004-07-03 2004.

  • Sætern, Ann Mari; Brandl, Martin. The solubilization potential of Hydroxypropyl-β-Cyclodextrin on Camptothecin. 2004 AAPS Annuel Meeting and Exposition 2004-11-07 - 2004-11-11 2004.

  • Sætern, Ann Mari; Flaten, Gøril Eide; Brandl, Martin. Incorporation Efficacy of Camptothecin into Liposomes. 2004 AAPS Annuel Meeting and Exposition 2004-11-07 - 2004-11-07 2004.

  • Sætern, Ann Mari; Skar, Merete L.; Braaten, Åsmund; Brandl, Martin. Hydrolysis of Phospholipids in Camptothecin containing liposomes. The Midnight Sun Meeting on Drug Transport and Drug Delivery 2004-07-01 - 2004-07-03 2004.

  • Nguyen, Nguyen B; Sætern, Ann Mari; Brandl, Martin. The influence of hydroxypropyl-ß-Cyclodextrine and pH on the solubility and lactone-carboxylate equlibrium of Camptothecin: A phase solubility study. 1st EUFEPS Conference on Optimising Drug Delivery and Formulation: New Challenges in Drug Delivery 2003-09-29 - 2003-10-01 2003.

  • Sætern, Ann Mari; Brandl, Martin. Preparation and characterization of a liposomal formulation for Camptothecin. 2001 AAPS Annual Meeting and Exposition 2001-10-21 - 2001-10-25 2001.

  • Sætern, Ann Mari; Bremer, Per Oscar; Hjelstuen, ole kristian. A one step kit for 99mTc-labelling of DNA-probes,. The Ninth European Symposium on Radiopharmacy and Radiopharmaceuticals 1999-03-06 - 1999-03-09 1999.

  • Sætern, Ann Mari. Utvikling av et preparasjonssett for marking av antisens oligonukleotider med Technetium-99m. 1999.

  • Medlem i forskergruppe