Forskningsinteresser

My research interest is to characterise and develop enzymes originating from the arctic marine environment for use in molecular biology and diagnostic applications. I have a particular research focus on nucleases and polymerases.

Arbeider i CRIStin


  • Hynes, Natasha; Furnes, Clemens; Fredriksen, Børge Nilsen; Winther, Tori; Bøgwald, Jarl; Larsen, Atle Noralf; Dalmo, Roy Ambli. Immune response of Atlantic salmon to recombinant flagellin. Vaccine 2011; Volum 29 (44). ISSN 0264-410X.s 7678 - 7687.s doi: 10.1016/j.vaccine.2011.07.138.

  • Inami, Makoto; Solem, Stein Tore; Jørgensen, Trond Ø.; Larsen, Atle Noralf. Characterization of an antiserum against Atlantic cod (Gadus morhua L.) g-type lysozyme. Fish and Shellfish Immunology 2010; Volum 29 (6). ISSN 1050-4648.s 1106 - 1109.s doi: 10.1016/j.fsi.2010.08.021.

  • Kumari, Jaya; Larsen, Atle Noralf; Bøgwald, Jarl; Dalmo, Roy Ambli. Interleukin-17D in Atlantic salmon (Salmo salar): Molecular characterization, 3D modelling and promoter analysis. Fish and Shellfish Immunology 2009; Volum 27 (5). ISSN 1050-4648.s 647 - 659.s doi: 10.1016/j.fsi.2009.08.009.

  • Larsen, Atle Noralf; Solstad, Terese; Svineng, Gunbjørg; Seppola, Marit; Jørgensen, Trond Ø.. Molecular characterisation of a goose-type lysozyme gene in Atlantic cod (Gadus morhua L.). Fish and Shellfish Immunology 2009; Volum 26 (1). ISSN 1050-4648.s 122 - 132.s doi: 10.1016/j.fsi.2008.03.021.

  • Helland, Ronny; Larsen, Renate Lie; Finstad, Solrun; Kyomuhendo, Peter; Larsen, Atle Noralf. Crystal structures of g-type lysozyme from Atlantic cod shed new light on substrate binding and the catalytic mechanism. Cellular and Molecular Life Sciences (CMLS) 2009; Volum 66 (15). ISSN 1420-682X.s 2585 - 2598.s doi: 10.1007/s00018-009-0063-x.

  • Seppola, Marit; Larsen, Atle Noralf; Steiro, Kari; Robertsen, Børre; Jensen, Ingvill. Characterisation and expression analysis of the interleukin genes, IL-1 beta, IL-8 and IL-10, in Atlantic cod (Gadus morhua L.). Molecular Immunology 2008; Volum 45. ISSN 0161-5890.s 887 - 897.s doi: 10.1016/j.molimm.2007.08.003.

  • Solstad, Terese; Larsen, Atle Noralf; Seppola, Marit; Jørgensen, Trond Ø.. Identification, cloning and expression analysis of a hepcidin cDNA of the Atlantic cod (Gadus morhua L.). Fish and Shellfish Immunology 2008; Volum 25 (3). ISSN 1050-4648.s 298 - 310.s doi: 10.1016/j.fsi.2008.05.013.

  • Helland, Ronny; Larsen, Atle Noralf; Smalås, Arne Oskar; Willassen, Nils Peder. The 1.8 angstrom crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species. The FEBS Journal 2006; Volum 273. ISSN 1742-464X.

  • Larsen, Atle Noralf; Moe, Elin; Helland, Ronny; Gjellesvik, Dag Rune; Willassen, Nils Peder. Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. The FEBS Journal 2006; Volum 273 (1). ISSN 1742-464X.s 47 - 60.

  • Piotrowski, Yvonne; Assefa, Netsanet Gizaw; Helland, Ronny; Johannessen, Kirsti Merete; Willassen, Nils Peder; Larsen, Atle Noralf. Enzymes from the Arctic - The cold gold. BioProsp 2015 2015-02-18 - 2015-02-20 2015.

  • Piotrowski, Yvonne; Assefa, Netsanet Gizaw; Helland, Ronny; Johannessen, Kirsti Merete; Landfald, Bjarne; Smalås, Arne O.; Jørgensen, Trond Ø.; Willassen, Nils Peder; Larsen, Atle Noralf. Enzymes from the Arctic - The cold gold. DNA polymerases: Biology, Diseases and Biomedical Applications 2014-08-31 - 2014-09-04 2014.

  • Piotrowski, Yvonne; Johannessen, Kirsti Merete; Assefa, Netsanet Gizaw; Landfald, Bjarne; Smalås, Arne O.; Jørgensen, Trond Ø.; Willassen, Nils Peder; Larsen, Atle Noralf. MabCent - Discovery of arctic marine enzymes. NBS kontaktmøte 2012 2012-01-19 - 2012-01-22 2012.

  • Larsen, Atle Noralf; Niiranen, Laila; Johannessen, Kirsti Merete; Finstad, Solrun; Jørgensen, Trond Ø.; Willassen, Nils Peder. MabCent - Hunting for Enzymes in the cold North. BioProsp 2009 2009-02-24 - 2009-02-26 2009.

  • Dalmo, Roy Ambli; Killie, jan-eirik; Schrøder, Merete Bjørgan; Larsen, Atle Noralf. Vil løse fiskesykdomsgåten. 2008.

  • Inami, Makoto; Larsen, Atle Noralf; rombout, jan; Schrøder, Merete Bjørgan; Viswanath, Kiron. A preliminary study on the intestinal immune system of Atlantic cod (Gadus morhua L.). The 7th Nordic symposium on Fish Immunology 2007-06-18 - 2007-06-22 2007.

  • Larsen, Atle Noralf. CodImm- Defence genes and immune mechanisms against viral and bacterial diseases of Atlantic cod. Avances en immunomodulacion de salmones 2007-11-28 - 2007-11-29 2007.

  • Larsen, Atle Noralf; Helland, Ronny; Svineng, Gunbjørg; Jørgensen, Trond Ø.. Identification, cloning and recombinant expression of a goose-type lysozyme from Atlantic cod (Gadus Morhua). The 7th Nordic symposium on Fish Immunology 2007-06-18 - 2007-06-22 2007.

  • Helland, Ronny; Larsen, Atle Noralf; Willassen, Nils Peder. Structural basis for substrate specificity in two enzymes in the Proteinase K family. 1st ESF and MAX-INF2 Course on Membrane Proteins. The rocky road from gene to structure. Oeiras, Portugal 2005-09-19 - 2005-09-25 2005.

  • Larsen, Atle Noralf; Helland, Ronny; Gjellesvik, Dag Rune; Willassen, Nils Peder. Structural basis for S1-S4 specificity in two enzymes of the proteinase K family. Bioprosp 2004 2004-10-13 - 2004-10-14 2004.

  • Publikasjoner utenfor Cristin

    Commercially available enzyme products from our research:

    Heat-labile exonuclease I: https://arcticzymes.com/technology/hl-exol/

    DNA polymerase – Isopol™ : https://arcticzymes.com/technology/isopol-dna-polymerase/

    DNA polymerase - Isopol™ SD+: https://arcticzymes.com/technology/isopol-sd/

    DNA polymerase - IsoPol™ BST+: https://arcticzymes.com/technology/isopol-bst/

    Patents:

    Larsen A.N., Piotrowski Y., “Position based increase in application relevant properties”. Patent application filed in UK December 2017

    Larsen A.N., Piotrowski Y., Assefa N.G., Lanes O. ”Marine DNA polymerase”. Patent application filed in UK March 2016, Patent Application No. 1604876.1

    Larsen A.N., Piotrowski Y., Willassen N.P., Lanes O., Lorentzen M.S., Solstad T., Andreassen E., Elde M. ”Heat Labile Exonucleases” International Patent Application, PCT/EP2015/001703

    Medlem i forskergruppe