Arktisk og marin biologi
Parasitic plants live as parasites on and from other plants. This creates huge losses in agriculture when a field of crop plants is infected. Knowledge on the development of these plants is therefore of high importance.
So far, many aspects around parasitic plant/host plant interactions and the chemical warfare that unfolds during attack and defence between them are unknown. We have over the last years developed a set of tools for the parasitic plant genus Cuscuta spp. (dodder) that allow us to approach the molecular mechanisms underlying host plant/parasitic plant interactions.
Parasitic plants use hydrolytic enzymes to break down cell walls of their host plants but are able to protect their own cell walls from enzymatic attack.
One of our goals is to study the difference between cell wall composition in the parasite Cuscuta and its hosts and analyze the enzymes involved. This knowledge can help to develop novel, more specific enzyme cocktails for plant biomass hydrolysis.
Plant cells are divided into "rooms" called compartments or organelles. The compartment that is typical for plant cells are the plastids (or chloroplasts). Plastids possess their own genetic information (the plastid genome or plastome) that contains important information for the biosynthetic pathways located in this compartment.
Most genes on the plastid genome are highly conserved. Different ecological niches can, however, lead to altered evolutionary pressures that, in return, can leave their "footprints" on the plastid genomes. We have examined plastid genomes from the parasitic plant genus Cuscuta and from a thermophilic red alga, Galdieria sulphuraria with respect to signs of altered evolutionary signatures.
The genetic information in plant cells is distributed between three compartments (nucleus, plastids and mitochondria). To function properly, these genomes are need to communicate with each other to synchronize their expression.
We are investigating how DNA binding proteins that are targeted to the nucleus and to the plastids contribute to the mutual communication between the plant genomes. We focus on thir role in the regulation fo plastid gene expression and their network of interactions in the chloroplast transcriptionally active chomosomes (TAC).
- Regulatory networks in plant cells: The role of dually targeted transcription factors for the development of cells and organisms. Financed as YFF project
(Yngre Fremragende Forskere = Outstanding Young Investigators) by
The Research Council of Norway.
- Plastids as reservoir for nuclear transcription factors. Financed as FRIBIO-project by
The Research Council of Norway.
- Plant cell wall degrading enzymes of the parasitic plant Cuscuta. PhD project financed by the University of Tromsø
- Plant cell wall degrading enzymes of parasitic plants as potential tools for biofuel poduction. Project financed by Tromsø Forskningsstiftelse/Mohn Foundation.
- Exploring the surface of crop plants as natural source for noxious weed repellents. Project financed by MABIT
Diploma in Biology 1994 from the University of Hamburg, Germany.
1995-2000 PhD student at the Universities of Cologne and Kiel.
2000-2002 Postdoc at the University of Arizona, USA.
2002-2006 Research Assisant at the University of Kiel, Germany. Habilitation 2006.
since November 2006 - Researcher/Professor at the Department of Biology, University of Tromsø, Norway.
since November 2010 - Leader of the Molecular Environments Research Group at the Department for Arctic and Marine Biology, Tromsø, Norway.
Plant innate immunity/macroautophagy
- Svenning S, Lamark T., Krause K., Johansen T (2011) Plant NBR1 is a selective autophagy substrate and a functional hybrid of the mammalian autophagic adapters NBR1 and p62/SQSTM1. Autophagy 7, 993-1010
Parasitic plant plastids:
- Krause K (2011) Piecing together the puzzle of plastome evolution. Planta 234, 647-656
- Tillich M, Krause K (2010) The ins and outs of editing and splicing of plastid RNAs: lessons from parasitic plants. New Biotechnology 27, 256-266
- Krause K (2008) From chloroplasts to "cryptic" plastids: evolution of plastid genomes in parasitic plants. Curr Genet 54, 111-121
- Funk H, Berg S, Krupinska K, Maier U, Krause K (2007) Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii. BMC Plant Biol. 7:45
- Berg S, Krupinska K, Krause K (2003) Plastids of three Cuscuta species differing in plastid coding capacity have a common parasite-specific RNA composition. Planta 218, 135-142
- van der Kooij, Krause K, Dörr I, Krupinska K (2000) Molecular, functional and ultrastructural characterisation of plastids from six species of the parasitic flowering plant genus Cuscuta. Planta 210, 701-707
Plastid gene expression and dually targeted transcription factors:
- Krause, K. (2015) Grand-scale theft: Kleptoplasty in parasitic plants?. Trends in Plant Science 20, 1360-1385
- Fuss, J.; Liegmann, O.; Krause, K.; Rensing, S.A. (2013) Green Targeting Predictor and Ambiguous Targeting Predictor 2: the pitfalls of plant protein targeting prediction and of transient protein expression in heterologous systems. New Phytologist 200, 1022-1033
- Krupinska, K.; Melonek, J.; Krause, K.(2013) New insights into plastid nucleoid structure and functionality. Planta 237, 653-664
- Krause, K.; Scharff, L. (2013) Reduced genomes from parasitic plant plastids: templates for minimal plastomes? Progress in Botany 75, 97 - 115
- Krause, K. and Krupinska, K. (2009) Nuclear regulators with a second home in organelles. Trends Plant Sci 14, 194-199
- Schwacke R Fischer K, Ketelsen B, Krupinska K, Krause K (2007) Comparative survey of plastid and mitochondrial targeting properties of transcription factors in Arabidopsis and rice. Mol. Genet. Genom. 277, 631-646
- Krause K, Kilbienski I, Mulisch M, Rödiger A, Schäfer A, Krupinska K (2005) DNA-binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles. FEBS Lett. 579, 3707-12
- Legen J, Kemp S, Krause K, Profanter B, Herrmann RG, Maier RM (2002) Comparative analysis of plastid transcription profiles of entire plastid chromosomes from tobacco attributed to wild-type and PEP-deficient transcription machineries. Plant J. 31, 171-188
Plastids of algae:
- Jain, K.; Krause, K.; Grewe, F.; Nelson, G.F; Weber, A.P.M; Christensen, A.C.; Mower, J.P. (2015) Extreme Features of the Galdieria sulphuraria Organellar Genomes: A Consequence of Polyextremophily?. Genome Biology and Evolution 7, 367-380
- Thangaraj, B., Ryan, C.M., Souda, P., Krause, K., Faull, K.F. Weber, A.P.M., Fromme, P. and Whitelegge J.P. (2010) Dta-directed top-down Fourier-transform mass spectrometry of a large integral membrane protein complex: Photosystem II from Galdieria sulphuraria. Proteomics, 10, 1-13
- Vanselow, C., Weber, A.P, Krause, K. and Fromme, P. (2009) Genetic analysis of the photosystem I subunits from the red alga, Galdieria sulphuraria. Biochim Biophys Acta 1787, 46-59
Prizes and Awards
Research Award 2011 from the Mohn Foundation, Tromsø, for the project "Plant cell wall degrading enzymes of parasitic plants as potential tools for biofuel production"
Energy-Award 2010 (Energipris 2010) rewarded by the Oil- and Energy-department in collaboration with FME-tankesmia
Outstanding Young Investigators Award 2007 (YFF) from The Research Council of Norway for the project “The role of dually targeted transcription factors for the development of cells and organisms”