/ UiT Norges arktiske universitet
 

Kirsten Krause

Krause, Kirsten


Professor
Arktisk og marin biologi


Telefon: 776 46415
Mobil jobb / Mobil privat: /
Epost : kirsten.krause@uit.no
Campus: Tromsø
Kontor/besøksadresse : NATURF 2114 / Naturfagbygget
Funksjon : Professor

Forskningsinteresser

Research Interests:

Parasitic plant/host plant interactions


Parasitic plants live as parasites on and from other plants. This creates huge losses in agriculture when a field of crop plants is infected. Knowledge on the development of these plants is therefore of high importance.
So far, many aspects around parasitic plant/host plant interactions and the chemical warfare that unfolds during attack and defence between them are  unknown. We have over the last years developed a set of tools for the parasitic plant genus Cuscuta spp. (dodder) that allow us to approach the molecular mechanisms underlying host plant/parasitic plant interactions.

Cell wall degrading enzymes from parasitic plants


Parasitic plants use hydrolytic enzymes to break down cell walls of their host plants but are able to protect their own cell walls from enzymatic attack. 
One of our goals is to study the difference between cell wall composition in the parasite Cuscuta and its hosts and analyze the enzymes involved. This knowledge can help to develop novel, more specific enzyme cocktails for plant biomass hydrolysis.

Evolution of plastid genomes


Cuscuta plastidPlant cells are divided into "rooms" called compartments or organelles. The compartment that is typical for plant cells are the plastids (or chloroplasts). Plastids possess their own genetic information (the plastid genome or plastome) that contains important information for the biosynthetic pathways located in this compartment.
Most genes on the plastid genome are highly conserved. Different ecological niches can, however, lead to altered evolutionary pressures that, in return, can leave their "footprints" on the plastid genomes. We have examined plastid genomes from the parasitic plant genus Cuscuta and from a thermophilic red alga, Galdieria sulphuraria with respect to signs of altered evolutionary signatures.

Integration of plastids into the regulatory network of plant cells


Dually targeted protein The genetic information in plant cells is distributed between three compartments (nucleus, plastids and mitochondria). To function properly, these genomes are need to communicate with each other to synchronize their expression.
We are investigating how DNA binding proteins that are targeted to the nucleus and to the plastids contribute to the mutual communication between the plant genomes. We focus on thir role in the regulation fo plastid gene expression and their network of interactions in the chloroplast transcriptionally active chomosomes (TAC).

Forskningsprosjekter

Funding:

- Regulatory networks in plant cells: The role of dually targeted transcription factors for the development of cells and organisms. Financed as YFF project
(Yngre Fremragende Forskere = Outstanding Young Investigators)
by
The Research Council of Norway
.

- Plastids as reservoir for nuclear transcription factors. Financed as FRIBIO-project by
The Research Council of Norway
.

- Plant cell wall degrading enzymes of the parasitic plant Cuscuta. PhD project financed by the University of Tromsø

- Plant cell wall degrading enzymes of parasitic plants as potential tools for biofuel poduction. Project financed by Tromsø Forskningsstiftelse/Mohn Foundation.

CV

Diploma in Biology 1994 from the University of Hamburg, Germany.

1995-2000 PhD student at the Universities of Cologne and Kiel.

2000-2002 Postdoc at the University of Arizona, USA.

2002-2006 Research Assisant at the University of Kiel, Germany. Habilitation 2006.

since November 2006 - Researcher/Professor at the Department of Biology, University of Tromsø, Norway.

since November 2010 - Leader of the Molecular Environments Research Group at the Department for Arctic and Marine Biology, Tromsø, Norway.

Andre publikasjoner

Selected publications:

Plant innate immunity/macroautophagy
- Svenning S, Lamark T., Krause K., Johansen T (2011) Plant NBR1 is a selective autophagy substrate and a functional hybrid of the mammalian autophagic adapters NBR1 and p62/SQSTM1. Autophagy 7, 993-1010

Parasitic plant plastids:
- Krause K (2011) Piecing together the puzzle of plastome evolution. Planta 234, 647-656
- Tillich M, Krause K (2010) The ins and outs of editing and splicing of plastid RNAs: lessons from parasitic plants. New Biotechnology 27, 256-266
- Krause K (2008) From chloroplasts to "cryptic" plastids: evolution of plastid genomes in parasitic plants. Curr Genet 54, 111-121
- Funk H, Berg S, Krupinska K, Maier U, Krause K (2007) Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii. BMC Plant Biol. 7:45
- Berg S, Krupinska K, Krause K (2003) Plastids of three Cuscuta species differing in plastid coding capacity have a common parasite-specific RNA composition. Planta 218, 135-142
- van der Kooij, Krause K, Dörr I, Krupinska K (2000) Molecular, functional and ultrastructural characterisation of plastids from six species of the parasitic flowering plant genus Cuscuta. Planta 210, 701-707

Plastid gene expression and dually targeted transcription factors:
- Krause, K. and Krupinska, K. (2009) Nuclear regulators with a second home in organelles. Trends Plant Sci 14, 194-199
-Krause, K., Herrmann, U., Fuss, J., Miao, Y. and Krupinska, K. (2009) Whirly proteins as communicators between plant organelles and the nucleus? Endocytobiosis Cell Res 19, 51-62
- Schwacke R Fischer K, Ketelsen B, Krupinska K, Krause K (2007) Comparative survey of plastid and mitochondrial targeting properties of transcription factors in Arabidopsis and rice. Mol. Genet. Genom. 277, 631-646
- Krause K, Kilbienski I, Mulisch M, Rödiger A, Schäfer A, Krupinska K (2005) DNA-binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles. FEBS Lett. 579, 3707-12
- Legen J, Kemp S, Krause K, Profanter B, Herrmann RG, Maier RM (2002) Comparative analysis of plastid transcription profiles of entire plastid chromosomes from tobacco attributed to wild-type and PEP-deficient transcription machineries. Plant J. 31, 171-188

Plastids of algae:
- Thangaraj, B., Ryan, C.M., Souda, P.,  Krause, K., Faull, K.F. Weber, A.P.M., Fromme, P. and Whitelegge J.P. (2010) Dta-directed top-down Fourier-transform mass spectrometry of a large integral membrane protein complex: Photosystem II from Galdieria sulphuraria. Proteomics, 10, 1-13
- Vanselow, C., Weber, A.P, Krause, K. and Fromme, P. (2009) Genetic analysis of the photosystem I subunits from the red alga, Galdieria sulphuraria. Biochim Biophys Acta 1787, 46-59

Priser og utmerkelser

Prizes and Awards

Research Award 2011 from the Mohn Foundation, Tromsø, for the project "Plant cell wall degrading enzymes of parasitic plants as potential tools for biofuel production"

Energy-Award 2010 (Energipris 2010) rewarded by the Oil- and Energy-department in collaboration with FME-tankesmia

Outstanding Young Investigators Award 2007 (YFF) from The Research Council of Norway for the project “The role of dually targeted transcription factors for the development of cells and organisms”

 

Hjemmesider

http://uit.no/ansatte/organisasjon/artikkel?p_menu=28713&p_document_id=222570&p_lang=1&p_dimension_id=88165

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Arbeider i CRIStin

  • Risan Johnsen, Hanne; Krause, Kirsten. Cellulase activity screening using pure carboxymethylcellulose: Application to soluble cellulolytic samples and to plant tissue prints.. International Journal of Molecular Sciences 2014; Volum 15 (1). ISSN 1422-0067.s 830 - 838.s doi: 10.3390/ijms15010830.
  • Krupinska, Karin; Melonek, Joanna; Krause, Kirsten. New insights into plastid nucleoid structure and functionality. Planta 2013; Volum 237 (3). ISSN 0032-0935.s 653 - 664.s doi: 10.1007/s00425-012-1817-5.
  • Herrmann, Ullrich; Krupinska, Karin; Krause, Kirsten. Association of translation elongation factor EF-Tu with chloroplast transcriptionally active chromosomes. (fulltekst) Endocytobiosis and cell research 2013; Volum 24. ISSN 0256-1514.s 27 - 34.
  • Fuss, Janina; Liegmann, Oliver; Krause, Kirsten; Rensing, Stefan A.. Green Targeting Predictor and Ambiguous Targeting Predictor 2: the pitfalls of plant protein targeting prediction and of transient protein expression in heterologous systems. New Phytologist 2013. ISSN 0028-646X.s doi: 10.1111/nph.12433.
  • Krause, Kirsten; Scharff, Lars. Reduced genomes from parasitic plant plastids: templates for minimal plastomes?. Progress in Botany 2013; Volum 75. ISSN 0340-4773.s 97 - 115.s doi: 10.1007/978-3-642-38797-5_3.
  • Krause, Kirsten. Plastid Genomes of Parasitic Plants: A Trail of Reductions and Losses. Springer Publishing Company 2012 ISBN 978-3-642-22379-2.s 79 - 103.
  • Isemer, Rena; Krause, Kirsten; Grabe, Nils; Kitahata, Nobutaka; Asami, Tadao; Krupinska, Karin. Plastid located WHIRLY1 enhances the responsiveness of Arabidopsis seedlings toward abscisic acid. Frontiers in Plant Science 2012; Volum 3 (283). ISSN 1664-462X.s doi: 10.3389/fpls.2012.00283.
  • Krause, Kirsten; Oetke, Svenja; Krupinska, Karin. Dual Targeting and Retrograde Translocation: Regulators of Plant Nuclear Gene Expression Can Be Sequestered by Plastids. International Journal of Molecular Sciences 2012; Volum 13 (9). ISSN 1422-0067.s 11085 - 11101.s doi: 10.3390/ijms130911085.
  • Svenning, Steingrim; Lamark, Trond; Krause, Kirsten; Johansen, Terje. Plant NBR1 is a selective autophagy substrate and a functional hybrid of the mammalian autophagic adapters NBR1 and p62/SQSTM1. Autophagy 2011; Volum 7 (9). ISSN 1554-8627.s 993 - 1010.s doi: 10.4161/auto.7.9.16389.
  • Krause, Kirsten. Piecing together the puzzle of parasitic plant plastome evolution. Planta 2011; Volum 234 (4). ISSN 0032-0935.s 647 - 656.s doi: 10.1007/s00425-011-1494-9.
  • Thangaraj, Balakumar; Ryan, Christopher M.; Souda, Puneet; Krause, Kirsten; Faull, Kym F.; Weber, Andreas P.M.; Fromme, Petra; Whitelegge, Julian P.. Data-directed top-down Fourier-transform mass spectrometry of a large integral membrane protein complex: Photosystem II from Galdieria sulphuraria. Proteomics 2010; Volum 10 (20). ISSN 1615-9853.s 3644 - 3656.s doi: 10.1002/pmic.201000190.
  • Tillich, Michael; Krause, Kirsten. The ins and outs of editing and splicing of plastid RNAs: lessons from parasitic plants. New Biotechnology 2010; Volum 27 (3). ISSN 1871-6784.s 256 - 266.s doi: 10.1016/j.nbt.2010.02.020.
  • Krause, Kirsten; Krupinska, Karin. Nuclear regulators with a second home in organelles. Trends in Plant Science 2009; Volum 14 (4). ISSN 1360-1385.s 194 - 199.s doi: 10.1016/j.tplants.2009.01.005.
  • Krause, Kirsten; Herrmann, Ullrich; Fuss, Janina; Miao, Ying; Krupinska, Karin. Whirly proteins as communicators between plant organelles and the nucleus?. Endocytobiosis and cell research 2009; Volum 19. ISSN 0256-1514.s 51 - 62.
  • Vanselow, Christopher; Weber, Andreas P.M.; Krause, Kirsten; Fromme, Petra. Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria. Biochimica et Biophysica Acta - Bioenergetics 2009; Volum 1787 (1). ISSN 0005-2728.s 46 - 59.s doi: 10.1016/j.bbabio.2008.10.004.
  • Krause, Kirsten. From chloroplasts to "cryptic" plastids: evolution of plastid genomes in parasitic plants. Current Genetics 2008; Volum 54. ISSN 0172-8083.s 111 - 121.s doi: 10.1007/s00294-008-0208-8.
  • Schwacke, R; Fischer, Karsten; Ketelsen, B; Krupinska, K; Krause, Kirsten. Comparative survey of plastid and mitochondrial targeting properties of transcription factors in Arabidopsis and rice. Molecular Genetics and Genomics 2007; Volum 277. ISSN 1617-4615.s 631 - 646.s doi: 10.1007/s00438-007-0214-4.
  • Funk, Helena; Berg, Sabine; Krupinska, Karin; Maier, Uwe G.; Krause, Kirsten. Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii. BMC Plant Biology 2007; Volum 7 (45). ISSN 1471-2229.s doi: 10.1186/1471-2229-7-45.
  • Brosch, Mario; Krause, Kirsten; Falk, Jon; Krupinska, Karin. Analysis of gene expression in amyloplasts of potato tubers. Planta 2007; Volum 227 (1). ISSN 0032-0935.s 91 - 99.s doi: 10.1007/s00425-007-0597-9.
  • Krause, Kirsten. Plastid Genome Variation and Selective Constraint in Species of the Parasitic Plant Genus Cuscuta. 2010 ISBN 978-1-61761-485-9.
  • Krause, Kirsten. Forschen in der Fremde - Am nördlichen Zipfel. 2013.
  • Krause, Kirsten. Geruchssinn bei Schmarotzerplanzen?. Biologie in unserer Zeit 2013; Volum 3 (43). ISSN 0045-205X.s 144 - .
  • Krause, Kirsten. Obligate and facultative heterotrophy in the plant kingdom: How (differently) did it affect plastid genomes and plastid gene expression?. ISE-XII 2013 2013-08-18 - 2013-08-22 2013.
  • Olsen, Stian; Krause, Kirsten. Artificial induction of haustoria in the parasitic angiosperm Cuscuta: A molecular study of haustoriogenesis. Black Forest Summer School on Bioinformatics for Molecular Biologists 2013-09-10 - 2013-09-13 2013.
  • Olsen, Stian; Yin, Lan; Krause, Kirsten. The dually targeted transcription factor TF1 and its role in the expression of plastid- and nuclear-encoded photosynthesis genes. PlantBio 2012 2012-10-17 - 2012-10-18 2012.
  • Krause, Kirsten; Lund, leidulf; Olsen, Bjørn Runar. Snylteplanters bevegelse. 2012.
  • Ketelsen, Bernd; Olsen, Stian; Fischer, Karsten; Krause, Kirsten. Correlation between 16S/18S rDNA ratio and chloroplast copy numbers in cotyledons of Arabidopsis thaliana: use for assessment of the impact of cytokinin response factor 5 on chloroplast development.. Endocytobiosis and cell research 2012; Volum 22. ISSN 0256-1514.s 43 - 47.
  • Yin, Lan; Krause, Kirsten. Dual targeting of a eukaryotic transcription factor, At2g44940. XXIV SPPS Congress 2011-08-21 - 2011-08-25 2011.
  • Herrmann, Ullrich; Schwacke, Rainer; Fischer, Karsten; Krause, Kirsten. Crosstalk between nucleus and organelles by dually targeted transcription factors?. 47th Contact Meeting 2011-02-03 - 2011-02-06 2011.
  • Risan Johnsen, Hanne; Krause, Kirsten. Analysis of plant cell wall degrading enzymes of the parasitic plant Cuscuta spp.. 47th Contact Meeting 2011-02-03 - 2011-02-06 2011.
  • Fuss, Janina; Krause, Kirsten. Localization and interaction of plastid localized RNA-binding proteins. 10th Norwegian Arabidopsis Meeting 2011-12-01 - 2011-12-01 2011.
  • Fuss, Janina; Liegmann, Oliver; Rensing, Stefan A.; Krause, Kirsten. Protein targeting in the moss Physcomitrella patens and the angiosperm Arabidopsis thaliana - how compatible are transport systems after 450 Ma of divergent evolution?. The Norwegian Biochemical Society 47th Contact Meeting 2011-02-03 - 2011-02-06 2011.
  • Krause, Kirsten. Slik kan ugress bli nyttig. Dagavisen Nordlys 2011.
  • Fuss, Janina; Herrmann, Ullrich; Krause, Kirsten. Crosstalk between nucleus and organelles: Dual targeting of transcription factors exemplified by members of the SEBF-like, GeBP and C3H families of Arabidopsis. 20th International Conference on Arabidopsis Research (ICAR) 2009-06-30 - 2009-07-04 2009.
  • Krause, Kirsten. Plastid Genome Variation and Selective Constraint in Species of the Parasitic Plant Genus Cuscuta. Molecular Genetics of Chloroplasts and Mitochondria 2009-09-20 - 2009-09-23 2009.
  • Krause, Kirsten. Plastid Genomics of a Parasite. ISE-G 2009-09-23 - 2009-09-25 2009.
  • Didriksen, Alena; Krause, Kirsten. Chloroplast gene expression in the alga Vaucheria litorea. Molecular Genetics of Chloroplasts and Mitochondria 2009-09-20 - 2009-09-23 2009.
  • Olsen, Stian; Krause, Kirsten. Analysis of plastid DNA content in barley primary leaves using laser dissection microscopy. Molecular Genetics of Chloroplasts and Mitochondria 2009-09-20 - 2009-09-23 2009.
  • Ketelsen, Bernd; Herrmann, Ullrich; Schwacke, Rainer; Fischer, Karsten; Krause, Kirsten. Dually targeted transcription factors: New players in the regulation of chloroplast gene expression?. Gordon Research Conference on Chloroplasts and Mitochondira 2008-08-10 - 2008-08-15 2008.
  • Krause, Kirsten. Plastid genomes of parasitic flowering plant species. 7th Norwegian Arabidopsis Meeting 2008-10-28 - 2008.
  • Krause, Kirsten. Genexpression in Cuscuta: Exons, Introns und MatK. Annual ISE-G -Meeting 2008-10-06 - 2008-10-08 2008.
  • Funk, Helena; Maier, Uwe G.; Krause, Kirsten. Splicing and editing of transcripts in parasitic plants. 7th Norwegian Arabidopsis Meeting 2008-10-28 - 2008.
  • Krause, Kirsten. Herbicides against Malaria? An endosymbiotic organelle as target for unconventional drugs. Kvinneforskermaraton 2007 2007-09-22 - 2007.
  • Krause, Kirsten. Characterization of the tocochromanol content and composition of different species of the parasitic flowering plant genus Cuscuta. Poster 2007-06-03 - 2007-06-07 2007.
  • Krause, Kirsten. Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii. Foredrag 2007-06-03 - 2007-06-07 2007.
  • Krause, Kirsten. Plastid genomes of parasitic flowering plant species. Botanical Congress Hamburg 2007 2007-09-03 - 2007-09-07 2007.
  • Krause, Kirsten. Eukaryotic transcription factors with dual localisation in the nucleus and in plastids or mitochondria - an important link in nuclear/organelle crosstalk?. 10th International Colloquium on Endocytobiology and Symbiosis 2007-09-10 - 2007-09-13 2007.
  • Krause, Kirsten. Herbizide - neue Wirkstoffe gegen die Malaria?. Biologie in unserer Zeit 2007; Volum 37 (4). ISSN 0045-205X.s 228 - 234.s doi: 10.1002/biuz.200610339.
  • Medlem i forskningsgrupper:

    Molecular Environments