Universitetet i Tromsø - Startsida Universitetet i Tromsø - Startsida
 

Leiros, Hanna-Kirsti Schrøder

Forsker
Kjemi

Funksjon
Forsker, Strukturkjemi, Norstruct

Telefon
776 45706

Epost

Kontor/besøksadresse
Forskningsparken 3
FPARK G 213
Ansatte IK - Hanna-Kirsti Schrøder Leiros
Foto: Ukjent

Stillingsbeskrivelse

Krystallisering av proteiner. Samling av røntgen data på instituttet og ved synkrotroner. Struktur bestemmelse og protein struktur analyse.

Biofysisk karakterisering av proteiner.

Veiledning av studenter.


Biografiske data

Utenlands opphold:

1994: 6 måneder i Groningen, Nederland.

1999: 6 måneder ved EMBL i Grenoble, Frankrike.

2003-2005: Post Doc. ved ESRF i Grenoble, Frankrike.


Forskerprofil i Cristin

Forskningsinteresser

Protein krystallografi, protein struktur og funksjon, temperatur tilpassing hos enzymer, antibiotika resistens, biofysiske egenskaper, ITC (Isothermal titration calorimetry), DSC (DSC Differential Scanning Calorimetry).


Forskningsprosjekter

- Temperatur tilpassing hos enzymet Isocitrate Dehydrogenase (IDH).

- Quorum sensing i bakterier

- 5-Nitroimidazole antibiotika resistente proteiner

- Antibiotika resistente beta-laktamase enzymer



Arbeider i CRIStin

  • Sivertsen, Annfrid; Isaksson, Johan; Leiros, Hanna-Kirsti S.; Svenson, Johan; Svendsen, John Sigurd; Brandsdal, Bjørn Olav. Synthetic cationic antimicrobial peptides bind with their hydrophobic parts to drug site II of human serum albumin. BMC Structural Biology 2014; Volum 14 (4). ISSN 1472-6807.s doi: 10.1186/1472-6807-14-4.
  • Bjerga, Gro Elin Kjæreng; Lund, Bjarte Aarmo; Leiros, Hanna-Kirsti S.. A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement. Microbial Cell Factories 2014; Volum 13. ISSN 1475-2859.s 38 - .s doi: 10.1186/1475-2859-13-38.
  • Fu, Juan; Leiros, Hanna-Kirsti S.; De pascale, Donatella; Johnson, Kenneth; Blencke, Hans-Matti; Landfald, Bjarne. Functional and structural studies of a novel cold-adapted esterase from an Arctic intertidal metagenomic library. Applied Microbiology and Biotechnology 2013; Volum 97. ISSN 0175-7598.s 3965 - 3978.s doi: 10.1007/s00253-012-4276-9.
  • Leiros, Hanna-Kirsti S.; Flydal, Marte Innselset; Martinez, Aurora. Structural and thermodynamic insight into phenylalanine hydroxylase from the human pathogen Legionella pneumophila. FEBS Open Bio 2013; Volum 3. ISSN 2211-5463.s 370 - 378.s doi: 10.1016/j.fob.2013.08.006.
  • Purohit, Amit Anand; Johansen, Jostein a; Hansen, Hilde; Leiros, Hanna-Kirsti S.; Kashulin, Alexander; Karlsen, Christian; Smalås, Arne O.; Haugen, Peik; Willassen, Nils Peder. Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family. Journal of Applied Microbiology 2013; Volum 115 (3). ISSN 1364-5072.s 835 - 847.s doi: 10.1111/jam.12264.
  • Borra, Naga Pardha Saradhi; Samuelsen, Ørjan; Spencer, James; Walsh, Timothy R.; Lorentzen, marit sjo; Leiros, Hanna-Kirsti S.. Crystal Structures of Pseudomonas aeruginosa GIM-1: Active-Site Plasticity in Metallo-beta-Lactamases. Antimicrobial Agents and Chemotherapy 2013; Volum 57 (2). ISSN 0066-4804.s 848 - 854.s doi: 10.1128/AAC.02227-12.
  • Leiros, Hanna-Kirsti S.; Borra, Naga Pardha Saradhi; Brandsdal, Bjørn Olav; Edvardsen, Kine Susann Waade; Spencer, James; Walsh, Timothy R.; Samuelsen, Ørjan. Crystal Structure of the Mobile Metallo-beta-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157. Antimicrobial Agents and Chemotherapy 2012; Volum 56 (8). ISSN 0066-4804.s 4341 - 4353.s doi: 10.1128/AAC.00448-12.
  • Leiros, Hanna-Kirsti S.; Fedøy, Anita-Elin; Leiros, Ingar; Steen, Ida Helene. The complex structure of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila support a new active site locking mechanism. FEBS Open Bio 2012. ISSN 2211-5463.
  • Saradhi, Pardha; Leiros, Hanna-Kirsti S.; Ahmad, Rafi; Spencer, James; Leiros, Ingar; Walsh, Timothy R.; Sundsfjord, Arnfinn; Samuelsen, Ørjan. Structural and Computational Investigations of VIM-7: Insights into the Substrate Specificity of VIM Metallo-beta-Lactamases. Journal of Molecular Biology 2011; Volum 411 (1). ISSN 0022-2836.s 174 - 189.s doi: 10.1016/j.jmb.2011.05.035.
  • Pedersen, hege lynum; Ahmad, Rafi; Riise, EK; Leiros, Hanna-Kirsti S.; Hauglid, Stefan; Espelid, S; Brandsdal, Bjørn Olav; Leiros, Ingar; Willassen, Nils P; Haugen, Peik. Experimental and computational characterization of the ferric uptake regulator from Aliivibrio salmonicida (Vibrio salmonicida). Journal of Microbiology 2010; Volum 48 (2). ISSN 1225-8873.s 174 - 183.s doi: 10.1007/s12275-010-9199-5.
  • Leiros, Hanna-Kirsti S.; Brandsdal, Bjørn Olav; McSweeney, Seán M.. Biophysical characterization and mutational analysis of the antibiotic resistance protein NimA from Deinococcus radiodurans. Biochimica et Biophysica Acta - Proteins and Proteomics 2010; Volum 1804 (4). ISSN 1570-9639.s 967 - 976.s doi: 10.1016/j.bbapap.2010.01.010.
  • Niiranen, Laila Mirja; Altermark, Bjørn; Brandsdal, Bjørn Olav; Leiros, Hanna-Kirsti S.; Helland, Ronny; Smalås, Arne Oskar; Willassen, Nils Peder. Effects of salt on the kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae. The FEBS Journal 2008; Volum 275. ISSN 1742-464X.s 1593 - 1605.s doi: 10.1111/j.1742-4658.2008.06317.x.
  • Leiros, Hanna-Kirsti S.; Tedesco, Consiglia; McSweeney, Seán M.. High-resolution structure of the antibiotic resistance protein NimA from Deinococcus radiodurans. Acta Crystallographica. Section F : Structural Biology and Crystallization Communications 2008; Volum 64. ISSN 1744-3091.s 442 - 447.s doi: 10.1107/S1744309108009901.
  • Wang, Ellen; Koutsioulis, D.; Leiros, Hanna-Kirsti S.; Andersen, Ole Andreas; Bouriotis, V.; Hough, Edward; Heikinheimo, Pirkko a. Crystal structure of alkaline phosphatase from the Antarctic bacterium TAB5. Journal of Molecular Biology 2007; Volum 366. ISSN 0022-2836.s doi: 10.1016/j.jmb.2006.11.079.
  • Riise, Ellen Kristin; Lorentzen, marit sjo; Helland, Ronny; Smalås, Arne Oskar; Leiros, Hanna-Kirsti S.; Willassen, Nils Peder. The first structure of a cold-active catalase from Vibrio salmonicida at 1.96 angstrom reveals structural aspects of cold adaptation. Acta Crystallographica Section D: Biological Crystallography 2007; Volum 63. ISSN 0907-4449.s 135 - 148.s doi: 10.1107/S0907444906043812.
  • Leiros, Hanna-Kirsti S.; Pey, AL; Innselset, M; Innselset, Marte; Moe, Elin; Leiros, Ingar; Steen, IH; Martinez, A. Structure of phenylalanine hydroxylase from Colwellia psychrerythraea 34H, a monomeric cold active enzyme with local flexibility around the active site and high overall stability. Journal of Biological Chemistry 2007; Volum 282. ISSN 0021-9258.s doi: 10.1074/jbc.M610174200.
  • Fedøy, Anita-Elin; Fedoy, AE; Yang, Nannan; Martinez, A; Leiros, Hanna-Kirsti S.; Steen, IH. Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability. Journal of Molecular Biology 2007; Volum 372. ISSN 0022-2836.s 130 - 149.s doi: 10.1016/j.jmb.2007.06.040.
  • Leiros, Hanna-Kirsti S.; McSweeney, Sean. The crystal structure of DR2241 from Deinococcus radiodurans at 1.9 Å resolution reveals a multi-domain protein with structural similarity to chelatases but also with two additional novel domains. Journal of Structural Biology 2007; Volum 159. ISSN 1047-8477.s 92 - 102.
  • Leiros, Hanna-Kirsti S.; Timmins, J.; Ravelli, R.B.; McSweeney, Seán M.. Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?. Acta Crystallographica Section D: Biological Crystallography 2006; Volum 62. ISSN 0907-4449.s 125 - 132.
  • Timmins, Joanna; Leiros, Hanna-Kirsti S.; Leonard, Gordon; Leiros, Ingar; McSweeney, Sean. Crystal structure of maltooligosyltrehalose trehalohydrolase from Deinococcus radiodurans in complex with disaccharides. Journal of Molecular Biology 2005; Volum 347 (5). ISSN 0022-2836.s 949 - 963.
  • Weik, M.; Schreurs, A.M.; Leiros, Hanna-Kirsti S.; Zaccai, G.; Ravelli, R.B.; Gros, P.. Supercooled liquid-like solvent in trypsin crystals: implications for crystal annealing and temperature-controlled X-ray radiation damage studies.. Journal of Synchrotron Radiation 2005; Volum 12 (3). ISSN 0909-0495.s 310 - 317.
  • Leiros, Hanna-Kirsti S.; Kozielski-Stuhrmann, S.; Kapp, U; Terradot, L; Leonard, G.A; McSweeney, Seán M.. Structural basis of 5-nitroimidazole antibiotic resistance: The crystal structure of NimA from Deinococcus radiodurans.. Journal of Biological Chemistry 2004; Volum 279 (53). ISSN 0021-9258.s 55840 - 55849.
  • Leiros, Hanna-Kirsti S.; Leiros, Hanna Kirsti Schrøder; Brandsdal, Bjørn Olav; Andersen, Ole Andreas; Os, vibeke; Leiros, Ingar; Leiros, Ingar; Helland, Ronny; Otlewski, J.; Otlewski, Jacek; Willassen, Nils Peder; Smalås, Arne Oskar. Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements. Protein Science 2004; Volum 13. ISSN 0961-8368.s 1056 - 1070.
  • Ravelli, R.G.B.; Leiros, Hanna-Kirsti S.; Pan, B.; Caffrey, M.; McSweeney, Seán M.. Specific radiation damage can be used to solve macromolecular crystal structures. Structure 2003; Volum 11 (2). ISSN 0969-2126.s 217 - 214.
  • Heikinheimo, Pirkko a; Helland, Ronny; Leiros, Hanna-Kirsti S.; Leiros, Ingar; Karlsen, Solveig; Evjen, gry; Ravelli, Raimond; Schoehn, Guy; Ruigrok, Rob; McSweeney, Sean; Hough, Edward. The structure of the bovine lysosomal a-mannosidase suggests a novel mechanism for low pH activation.. Journal of Molecular Biology 2003; Volum 327 (3). ISSN 0022-2836.s 631 - 644.
  • Leiros, Hanna-Kirsti S.; McSweeney, Seán M.; Smalås, Arne Oskar. Atomic resolution structures of trypsin provide insight into structural radiation damage. Acta Crystallographica Section D: Biological Crystallography 2001; Volum 57. ISSN 0907-4449.s 488 - 497.
  • Smalås, Arne Oskar; Leiros, Hanna-Kirsti S.; Os, V.; Willassen, Nils Peder. Cold adapted Enzymes. Biotechnology Annual Review 2000; Volum 6. ISSN 1387-2656.s 1 - 57.
  • Gorfe, A.A.; Brandsdal, Bjørn Olav; Leiros, Hanna-Kirsti S.; Helland, Ronny; Smalås, Arne Oskar. Electrostatics of mesophilic and psychrophilic trypsin isoenzymes: qualitative evaluation of electrostatic differences at the substrate binding site. Proteins: Structure, Function, and Genetics 2000; Volum 40. ISSN 0887-3585.s 207 - 217.
  • Leiros, Hanna-Kirsti S.; Willassen, Nils Peder; Smalås, Arne Oskar. Residue determinants and sequence analysis of cold-adapted trypsins. Extremophiles 1999; Volum 3. ISSN 1431-0651.s 205 - 219.
  • Leiros, Hanna-Kirsti S.; Willassen, Nils Peder; Smalås, Arne Oskar. Structural comparison of psychrophilic and mesophilic trypsins. Elucidating the molecular basis of cold-adaptation. European Journal of Biochemistry 1999; Volum 267. ISSN 0014-2956.s 1039 - 1049.
  • Leiros, Hanna-Kirsti S.. On the Road Searching for Inhibitors against beta-Lactamases Involved in Antibiotic Resistance. The Norwegian Biochemical Society (NBS) Contact Meeting 2014 2014-01-23 - 2014-01-26 2014.
  • Skagseth, Susann; Carlsen, Trine Josefine Olsen; Bjerga, Gro Elin Kjæreng; Bayer, Annette; Samulesen, Ørjan; Leiros, Hanna-Kirsti S.. The importance of residue W228 and Y233 in the metallo-beta-lactamase GIM-1. The Norwegian Biochemical Society (NBS) Contact Meeting 2014 2014-01-23 - 2014-01-26 2014.
  • Skagseth, Susann; Edvardsen, Kine Susann Waade; Bjerga, Gro Elin Kjæreng; Lorentzen, marit sjo; Leiros, Ingar; Samulesen, Ørjan; Leiros, Hanna-Kirsti S.. Site-directed mutagenesis of metallo-beta-lactamase VIM-7 from the opportunistic human pathogenic bacteria Pseudomonas aeruginosa. NBS konferanse 2013-01-31 - 2013-02-03 2013.
  • Fonseca, Davi de Miranda; Baar, Cecilie Christensen; Assefa, Netsanet Gizaw; Moe, Elin; Liabakk, Nina-Beate; Leiros, Hanna-Kirsti S.; Aas, Per Arne; Skorpen, Frank; Krokan, Hans Einar. Thermo-sensitive human UNG-mutants. XII Annual SBDR Program Project Workshop 2013-04-11 - 2013-04-13 2013.
  • Ove Alexander, Høgmoen Åstrand; Edvardsen, Kine Susann Waade; Samuelsen, Ørjan; Leiros, Hanna-Kirsti S.; Bayer, Annette; Rongved, Pål. Compounds. 2013.
  • Leiros, Hanna-Kirsti S.. Metallo-beta-Lactamases Cause Clinical Problems due to Antibiotic Resistance. Can Crystal Structures Help Out?. Norsk Biokjemisk Selskap (NBS) 49th Contact Meeting 2013-01-31 - 2013-02-03 2013.
  • Leiros, Hanna-Kirsti S.. Radiation damage. BioStruct- Synchrotron Data Collection, ESRF 2013-09-09 - 2013-09-13 2013.
  • Fedøy, Anita-Elin; Leiros, Hanna-Kirsti S.; Steen, Ida Helene; Birkeland, Nils-Kåre. Bacterial subfamiliy II isocitrate dehydrogenase: adaptations to low and high temperatures. Extremophiles 2012 2012-09-10 - 2012-09-13 2012.
  • Leiros, Hanna-Kirsti S.. Antibiotikaresistent - hva nå. Science - who cares? 2010-09-02 - 2010.
  • Leiros, Hanna-Kirsti S.. Antibiotika resistens: en utfordring både for forskere og kliniske behandlere. Populærvitenskaplige fordragsserie (fulltekst) 2010-12-03 - 2010.
  • Leiros, Hanna-Kirsti S.; Pey, Angel L.; Innselset, M; Moe, Elin; Leiros, Ingar; Steen, Ida Helene; Martinez, Aurora. Crystal Structure of phenylalanine hydroxylase from Colwellia psychrerythraea 34H reveals a monomeric cold active enzyme with local flexibility around the active site and high overall stability. Norwegian Biochemical Society (NBS) contact meeting 2008 2008-01-17 - 2008-01-20 2008.
  • Fedøy, Anita-Elin; Yang, Nannan; Martinez, Aurora; Leiros, Hanna-kirsti S.; Steen, Ida Helene. A cold-active enzyme with an unusual high thermal stability: Isocitrate dehydrogenase from the psycrophilic bacterium Desulfotalea psycrophila. Extremophiles 2008 2008-09-07 - 2008-09-11 2008.
  • Leiros, Hanna-Kirsti S.. A structural view on antibiotic resistance: DrNimA (DR0842) from Deinococcus radiodurans. Combating Antibiotic Resistance-Development of Inhibitors against Antibiotic Resistance Enzymes. 2008-08-13 - 2008-08-13 2008.
  • Leiros, Hanna-Kirsti S.. Radiation Damage and Dose-Rate effects on heavy atoms.. Guest lecture at Department of Chemistry, University of Oslo, Norway. 2007-10-19 - 2007-10-19 2007.
  • Leiros, Hanna-Kirsti S.. Crystal Structure of phenylalanine hydroxylase from Colwellia psychrerythraea 34H - reveals a monomeric cold active enzyme with local flexibility around the active site and high overall stability. 9th International Conference on Biology and Synchrotron Radiation 2007-08-12 - 2007-08-17 2007.
  • Leiros, Hanna-Kirsti S.. Radiation damage: How to avid it while colleting X-ray data and how to use it solving new protein structures. 19th Nordic Structural Chemistry Meeting 2007-01-08 - 2007-01-11 2007.
  • Leiros, Hanna-Kirsti S.. Radiation damage: How to avoid it while collecting X-ray data and how to use it.. 19th Nordic Structural Chemistry Meeting, NSM 19 2007-01-08 - 2007-01-11 2007.
  • Leiros, Hanna-Kirsti S.. Phenylalanine hydroxylase from the cold adapted bacterium Colwellia psychrerythraea 34: - Biochemical and structural properties. NorStruct seminar at Sommarøy, Norway 2006-12-07 - 2006-12-08 2006.
  • Timmins, Joanna; Leiros, Hanna-Kirsti S.; Leonard, Gordon; Leiros, Ingar; McSweeney, Sean. Crystal Structure of Maltooligosyltrehalose Trehalohydrolase from D. radiodurans. ESRF Highlights 2005 2006.s 68 - 70.
  • Leiros, Hanna-Kirsti schrøder; McSweeney, Seán M.. Radiation Damage and Dose-Rate effects on heavy atoms. Gjesteforelesning 2005-10-19 - 2005-10-19 2005.
  • Leiros, Hanna-Kirsti S.. Is the Deinococcus radiodurans protein DR2241 a chelatase? Inserting Fe or Co2+ into Hemes?. ESRF Macro Molecular science day, Col de Porte, Grenoble, France. 2005-06-14 - 2005-06-14 2005.
  • Hall, David Richard; Leiros, Ingar; Leiros, Hanna-Kirsti S.; Micossi, E.; Gordon, E.; Macedo, S.; Kapp, U.; Jamin, C.; Timmins, Joanna; Leonard, Gordon; McSweeney, Sean. Structural Genomics at the ESRF: Studying Deinococcus radiodurans. ESRF Highlights 2003 2004.s 29 - 29.
  • Heikinheimo, Pirkko a; Helland, Ronny; Leiros, Hanna-Kirsti S.; Leiros, Ingar; Karlsen, Solveig; Evjen, gry; Ravelli, Raimond; Schoehn, Guy; Ruigrok, Rob; Tollersrud, ole kristian; McSweeney, Sean; Hough, Edward. Structure of the Bovine Lysosomal a-Mannosidase, the Enzyme Involved in the Lysosomal Storage Disease a-Mannosidosis. ESRF Highlights 2002 2003.s 12 - 13.
  • Leiros, Hanna-Kirsti S.. Comparison of psychrophilic and mesophilic trypsins : elucidating the basis of cold-adaptation. 1999.
  • Helland, Ronny; Berglund, Gunnar; Hansen, Lars Kr; Heimstad, Eldbjørg S.; Hordvik, Asbjørn; Leiros, Ingar; Leiros, Hanna-Kirsti S.; Os, vibeke; Outzen, Heidi; Willassen, Nils Peder; Smalås, Arne Oskar. Structure-Function Relation Studies of Enzymes from Cold Adapted Species.. Higher European Research Course for Users of Large Experimental Systems (HERCULES) 1995-02-26 - 1995-04-07 1995.
  • Leiros, Hanna-Kirsti S.. Crystallographic studies of cationic trypsin from the North Atlantic salmon (Salmo salar). 1995.
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