Universitetet i Tromsø - Startsida Universitetet i Tromsø - Startsida
 

Isaksson, Johan

Forsker
Kjemi

Funksjon
Forsker, IK, Managing the NMR lab, NMR specialist Smallstruct

Telefon
776 44842

Epost

Kontor/besøksadresse
Medisin og Helsefag
REALF C 206.2
JohanIsaksson2.png

Mobil privat
41354726

Alternativ Kontaktinfo

REALF C 206.2


Stillingsbeskrivelse

Responsible for the NMR lab and research scientist associated to the D3 (Drug Discovery and Design) initiative. Also NMR specialist in SmallStruct where I assist in structure elucidation and verification.

Teaching at the NMR course, KJE-3303/8303.


Forskningsinteresser

I have broad scientific interests within various applications of NMR spectroscopy, from small molecules to proteins. Structure/activity relationship studies are of particular interest (x-filtered transfer experiments, hydration, relaxation, dynamics). 

I have been working with NMR since 1998, using both Bruker and Varian/Agilent equipment. Working experience includes structure determinations of Nucleic acids and Proteins, structure verification/elucidation of small molecules in pharmaceutical industry and natural products from bioprospecting as well small molecules interaction with proteins and liposomes.


Forskningsprosjekter

Small synthetic anti-microbial peptides (SAMPs). There are ongoing studies of the basic properties of SAMPs in liposomes. Their mechanism of action is of particular interest and NMR data is combined with Molecular Dynamics simulations in lipid bilayers, in collaboration with Bjørn Olav Brandsdal, CTCC, Tromsø. Project completed.

Anti-microbial/anti-cancer peptides. Studies of the somewhat larger synthetic peptides in collaboration with Morten Strøm, IFA, Tromsø University are ongoing. Work has so far involved liposome interaction and HSA binding studies. Project completed.

dUTPase. Human dUTPase, which is relevant in cancer, tuberculosis and malaria, has been successfully assigned and its inherent dynamics in presence and absence of inhibitor is being studied by high field NMR. In collaboration with Tatiana Agback, Medivir AB, Stockholm, Patrik Lundström, Linköping University, Sweden and Peter Agback, SLU, Uppsala, Sweden.

MMP12. Protein hydration studies of MMP12 (metalloprotease) in collaboration with Tatiana Agback, Medivir AB, Stockholm, Sweden, and Helena Kovacs, Bruker Spectrospin, Fällanden, Switzerland. Project completed.

NBR1. Protein studies of the Ubiquitin binding domain and its regulation using NMR spectroscopy, in collaboration with the Terje Johansen, Tromsø University.

PKA/Au-kinases/ABL. Kinase inhibitor projects are in the start-up phase in collaboration with Richard Engh, Norstruct, Tromsø University and John S. Svendsen, Lytix Biopharma, Tromsø. Fragment screening and isotope labelling strategies are to be employed in search of new kinase inhibitors, and hits are characterized in terms of binding orientation.

Theoretic prediction of NMR parameters. In collaboration with Kenneth Ruud and Kathrin Hopmann, methods for accurately simulating chemical shifts near halogens and/or metals, and long-range coupling constants are employed to answer stuctural questions for small organic molecules.


Undervisning

Teaching in the NMR course, KJE-3303/8303.


CV
2011- Research and NMR site management, Tromsø University, Norway
2009

PostDoc scientist for Prof. John Sigurd Svendsen in Organic chemistry (NMR), Tromsø University, Norway

2005

NMR Research scientist (pharmaceutic industry), Medivir AB, Sweden

2005

Ph.D. in Bioorganic chemistry (NMR), Uppsala University, Sweden

1998 M.Sc. in Biomedical chemistry, Kalmar University, Sweden

   

 

 

 



Arbeider i CRIStin

  • Kovacs, H; Agback, T; Isaksson, Johan. Probing water-protein contacts in a MMP-12/CGS27023A complex by nuclear magnetic resonance spectroscopy. Journal of Biomolecular NMR 2012; Volum 53 (2). ISSN 0925-2738.s 85 - 92.s doi: 10.1007/s10858-012-9624-7.
  • Isaksson, Johan; Nyström, Susanne; Derbyshire, Dean; Wallberg, Hans; Agback, Tatiana; Kovacs, Helena; Bertini, Ivano; Giachetti, Andrea; Luchinat, Claudio. Does a Fast Nuclear Magnetic Resonance Spectroscopy- and X-Ray Crystallography Hybrid Approach Provide Reliable Structural Information of Ligand-Protein Complexes? A Case Study of Metalloproteinases. Journal of Medicinal Chemistry 2009; Volum 52. ISSN 0022-2623.s 1712 - 1722.s doi: 10.1021/jm801388q.
  • Isaksson, Johan; Plashkevych, Oles; Pradeepkumar, P. I.; Chatterjee, S.; Barman, J.; Pathmasiri, W.; Shrivastava, P.; Petit, Catherine; Chattopadhyaya, Jyoti. Oxetane Locked Thymidine in the Dickerson-Drew Dodecamer Causes Local Base Pairing Distortions - An NMR Structure and Hydration Study. Journal of Biomolecular Structure and Dynamics 2005; Volum 23 (3). ISSN 0739-1102.s 299 - 330.
  • Isaksson, Johan; Chattopadhyaya, Jyoti. A Uniform Mechanism Correlating Dangling-End Stabilization and Stacking Geometry. Biochemistry 2005; Volum 44. ISSN 1520-4995.s 5390 - 5401.s doi: 10.1021/bi047414f.
  • Acharya, Sandipta; Barman, Jharna; Cheruku, Pradeep; Chatterjee, Subhrangsu; Acharya, Parag; Isaksson, Johan; Chattopadhyaya, Jyoti. Significant pKa Perturbation of Nucleobases Is an Intrinsic Property of the Sequence Context of DNA and RNA. Journal of the American Chemical Society 2004; Volum 126. ISSN 0002-7863.s 8674 - 8681.s doi: 10.1021/ja048484c.
  • Isaksson, Johan; Acharya, Sandipta; Barman, J.; Cheruku, Pradeep; Chattopadhyaya, Jyoti. Single-Stranded Adenine-Rich DNA and RNA Retain Structural Characteristics of Their Respective Double-Stranded Conformations and Show Directional Differences in Stacking Pattern. Biochemistry 2004; Volum 43. ISSN 1520-4995.s 15996 - 16010.s doi: 10.1021/bi048221v.
  • Boon, Elizabeth M.; Barton, Jacqueline K.; Pradeepkumar, P. I.; Isaksson, Johan; Petit, Catherine; Chattopadhyaya, Jyoti. An Electrochemical Probe of DNA Stacking in an Antisense Oligonucleotide Containing a C3'-endo-Locked Sugar. Angewandte Chemie International Edition 2002; Volum 41 (8). ISSN 1433-7851.s 3402 - 3405.
  • Zamaratski, Edouard; Trifonova, Anna; Acharya, Parag; Isaksson, Johan; Maltseva, Tatiana; Chattopadhyaya, Jyoti. Do the 16-mer, 5'-GUGGUCUGAUGAGGCC-3' and the 25-mer, 5'-GGCCGAAACUCGUAAGAGUCACCAC-3', Form a Hammerhead Ribozyme Structure in Physiological Conditions? An NMR and UV Thermodynamic Study. Nucleosides, Nucleotides & Nucleic Acids 2001; Volum 20. ISSN 1525-7770.s 1219 - 1223.
  • Isaksson, Johan; Zamaratski, Edouard; Maltseva, Tatiana; Agback, Peter; Kumar, A.; Chattopadhyaya, Jyoti. The First Example of a Hoogsteen Basepaired DNA Duplex in Dynamic Equilibrium with a Watson-Crick Basepaired Duplex - A Structural (NMR), Kinetic and Thermodynamic Study. Journal of Biomolecular Structure and Dynamics 2001; Volum 18 (6). ISSN 0739-1102.s 783 - 806.
  • Isaksson, Johan; Maltseva, Tatiana; Agback, Peter; Luo, X.; Kumar, A; Zamaratski, Edouard; Chattopadhyaya, Jyoti. A Single Carbocyclic Nucleotide Substitution in a 12mer DNA Gives a Hoogsteen Basepaired Duplex (Till 38°C) and a Hairpin (Till 65°C). A 600 MHz NMR Spectroscopic Study. Nucleosides, Nucleotides & Nucleic Acids 1999; Volum 18. ISSN 1525-7770.s 1593 - 1596.
  • Sivertsen, Annfrid; Isaksson, Johan; Leiros, Hanna-Kirsti S.; Svenson, Johan; Svendsen, John Sigurd; Brandsdal, Bjørn Olav. Synthetic cationic antimicrobial peptides bind with their hydrophobic parts to drug site II of human serum albumin. BMC Structural Biology 2014; Volum 14 (4). ISSN 1472-6807.s doi: 10.1186/1472-6807-14-4.
  • Sivertsen, Annfrid; Tørfoss, Veronika; Isaksson, Johan; Ausbacher, Dominik; Anderssen, Trude; Brandsdal, Bjørn Olav; Havelkova, Martina; Skjørholm, Anne Elisabeth; Strøm, Morten B.. Anticancer potency of small linear and cyclic tetrapeptides and pharmacokinetic investigations of peptide binding to human serum albumin. Journal of Peptide Science 2014. ISSN 1075-2617.s doi: 10.1002/psc.2615.
  • Li, Xiaojun; Hopmann, Kathrin Helen; Hudecova, Jana; Isaksson, Johan; Novotná, Jana; Stensen, Wenche; Andrushchenko, Valery; Urbanová, Marie; Svendsen, John Sigurd; Bour, Petr; Ruud, Kenneth. Determination of Absolute Configuration and Conformation of a Cyclic Dipeptide by NMR and Chiral Spectroscopic Methods. Journal of Physical Chemistry A 2013; Volum 117 (8). ISSN 1089-5639.s 1721 - 1736.s doi: 10.1021/jp311151h.
  • Olsen, Elisabeth Klungerbo; Hansen, Espen; Isaksson, Johan; Andersen, Jeanette hammer. Cellular Antioxidant Effect of Four Bromophenols from the Red Algae, Vertebrata lanosa. Marine Drugs 2013; Volum 11. ISSN 1660-3397.s 2769 - 2784.s doi: 10.3390/md11082769.
  • Demissie, Taye B.; Repisky, Michal; Komorovsky, Stanislav; Isaksson, Johan; Svendsen, John Sigurd; Dodziuk, H; Ruud, Kenneth. Four-component relativistic chemical shift calculations of halogenated organic compounds. Journal of Physical Organic Chemistry 2013; Volum 26 (8). ISSN 0894-3230.s 679 - 687.s doi: 10.1002/poc.3157.
  • Isaksson, Johan; Woestenenk, Esmeralda; Sahlberg, Christer; Agback, Tatiana. Backbone nuclear magnetic resonance assignment of human deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase). Biomolecular NMR Assignments 2013. ISSN 1874-2718.s 1 - 4.s doi: 10.1007/s12104-013-9457-7.
  • Østnes Hanssen, Kine; Schuler, Bruno; Williams, Antony J.; Demissie, Taye B.; Hansen, Espen; Andersen, Jeanette hammer; Svenson, Johan; Blinov, Kirill; Repisky, Michal; Mohn, Fabian; Meyer, Gerhard; Svendsen, John Sigurd; Ruud, Kenneth; Elyashberg, Mikhail; Gross, Leo; Jaspars, Marcel; Isaksson, Johan. A Combined Atomic Force Microscopy and Computational Approach for the Structural Elucidation of Breitfussin A and B: Highly Modified Halogenated Dipeptides from Thuiaria breitfussi. Angewandte Chemie International Edition 2012; Volum 51 (49). ISSN 1433-7851.s 12238 - 12241.s doi: 10.1002/anie.201203960.
  • Kovacs, H; Agback, T; Isaksson, Johan. Probing water-protein contacts in a MMP-12/CGS27023A complex by nuclear magnetic resonance spectroscopy. Journal of Biomolecular NMR 2012; Volum 53 (2). ISSN 0925-2738.s 85 - 92.s doi: 10.1007/s10858-012-9624-7.
  • Tørfoss, Veronika; Isaksson, Johan; Ausbacher, Dominik; Brandsdal, Bjørn Olav; Flaten, Gøril Eide; Anderssen, Trude; Cavalcanti Jacobsen, Cristiane de Albuquerque; Havelkova, Martina; Nguyen, Leonard T.; Vogel, Hans J.; Strøm, Morten B.. Improved anticancer potency by head-to-tail cyclization of short cationic anticancer peptides containing a lipophilic b2,2-amino acid. Journal of Peptide Science 2012; Volum 18 (10). ISSN 1075-2617.s 609 - 619.s doi: 10.1002/psc.2441.
  • Isaksson, Johan; Brandsdal, Bjørn Olav; Engqvist, Stig Olov Magnus; Flaten, Gøril Eide; Svendsen, John Sigurd; Stensen, Wenche. A Synthetic Antimicrobial Peptidomimetic (LTX 109): Stereochemical Impact on Membrane Disruption. Journal of Medicinal Chemistry 2011; Volum 54 (16). ISSN 0022-2623.s 5786 - 5795.s doi: 10.1021/jm200450h.
  • Isaksson, Johan; Nyström, Susanne; Derbyshire, Dean; Wallberg, Hans; Agback, Tatiana; Kovacs, Helena; Bertini, Ivano; Giachetti, Andrea; Luchinat, Claudio. Does a Fast Nuclear Magnetic Resonance Spectroscopy- and X-Ray Crystallography Hybrid Approach Provide Reliable Structural Information of Ligand-Protein Complexes? A Case Study of Metalloproteinases. Journal of Medicinal Chemistry 2009; Volum 52. ISSN 0022-2623.s 1712 - 1722.s doi: 10.1021/jm801388q.
  • Isaksson, Johan; Plashkevych, Oles; Pradeepkumar, P. I.; Chatterjee, S.; Barman, J.; Pathmasiri, W.; Shrivastava, P.; Petit, Catherine; Chattopadhyaya, Jyoti. Oxetane Locked Thymidine in the Dickerson-Drew Dodecamer Causes Local Base Pairing Distortions - An NMR Structure and Hydration Study. Journal of Biomolecular Structure and Dynamics 2005; Volum 23 (3). ISSN 0739-1102.s 299 - 330.
  • Isaksson, Johan; Chattopadhyaya, Jyoti. A Uniform Mechanism Correlating Dangling-End Stabilization and Stacking Geometry. Biochemistry 2005; Volum 44. ISSN 1520-4995.s 5390 - 5401.s doi: 10.1021/bi047414f.
  • Acharya, Sandipta; Barman, Jharna; Cheruku, Pradeep; Chatterjee, Subhrangsu; Acharya, Parag; Isaksson, Johan; Chattopadhyaya, Jyoti. Significant pKa Perturbation of Nucleobases Is an Intrinsic Property of the Sequence Context of DNA and RNA. Journal of the American Chemical Society 2004; Volum 126. ISSN 0002-7863.s 8674 - 8681.s doi: 10.1021/ja048484c.
  • Isaksson, Johan; Acharya, Sandipta; Barman, J.; Cheruku, Pradeep; Chattopadhyaya, Jyoti. Single-Stranded Adenine-Rich DNA and RNA Retain Structural Characteristics of Their Respective Double-Stranded Conformations and Show Directional Differences in Stacking Pattern. Biochemistry 2004; Volum 43. ISSN 1520-4995.s 15996 - 16010.s doi: 10.1021/bi048221v.
  • Boon, Elizabeth M.; Barton, Jacqueline K.; Pradeepkumar, P. I.; Isaksson, Johan; Petit, Catherine; Chattopadhyaya, Jyoti. An Electrochemical Probe of DNA Stacking in an Antisense Oligonucleotide Containing a C3'-endo-Locked Sugar. Angewandte Chemie International Edition 2002; Volum 41 (8). ISSN 1433-7851.s 3402 - 3405.
  • Zamaratski, Edouard; Trifonova, Anna; Acharya, Parag; Isaksson, Johan; Maltseva, Tatiana; Chattopadhyaya, Jyoti. Do the 16-mer, 5'-GUGGUCUGAUGAGGCC-3' and the 25-mer, 5'-GGCCGAAACUCGUAAGAGUCACCAC-3', Form a Hammerhead Ribozyme Structure in Physiological Conditions? An NMR and UV Thermodynamic Study. Nucleosides, Nucleotides & Nucleic Acids 2001; Volum 20. ISSN 1525-7770.s 1219 - 1223.
  • Isaksson, Johan; Zamaratski, Edouard; Maltseva, Tatiana; Agback, Peter; Kumar, A.; Chattopadhyaya, Jyoti. The First Example of a Hoogsteen Basepaired DNA Duplex in Dynamic Equilibrium with a Watson-Crick Basepaired Duplex - A Structural (NMR), Kinetic and Thermodynamic Study. Journal of Biomolecular Structure and Dynamics 2001; Volum 18 (6). ISSN 0739-1102.s 783 - 806.
  • Isaksson, Johan; Maltseva, Tatiana; Agback, Peter; Luo, X.; Kumar, A; Zamaratski, Edouard; Chattopadhyaya, Jyoti. A Single Carbocyclic Nucleotide Substitution in a 12mer DNA Gives a Hoogsteen Basepaired Duplex (Till 38°C) and a Hairpin (Till 65°C). A 600 MHz NMR Spectroscopic Study. Nucleosides, Nucleotides & Nucleic Acids 1999; Volum 18. ISSN 1525-7770.s 1593 - 1596.
  • Hanssen, Kine Ø.; Schuler, Bruno; Williams, Antony J.; Demissie, Taye B.; Hansen, Espen; Andersen, Jeanette hammer; Svenson, Johan; Svendsen, John Sigurd; Gross, Leo; Jaspars, Marcel; Isaksson, Johan. Introducing Atomic Force Microscopy in Structure Elucidation. SMASH 2013 2013-09-22 - 2013-09-25 2013.
  • Olsen, Elisabeth Klungerbo; Hansen, Espen; Isaksson, Johan; Andersen, Jeanette hammer. Antioxidant effect of four bromophenols from the red algae Vertebrata lanosa. International Congress on Natural Products Research 2012-07-28 - 2012-08-01 2012.
  • Olsen, Elisabeth; Hansen, Espen; Isaksson, Johan; Andersen, Jeanette hammer. Antioxidant effect of four bromophenols from the red algae Vertebrata lanosa. Planta Medica 2012; Volum 78 (11). ISSN 0032-0943.s 1146 - 1146.
  • Isaksen, Geir Villy; Isaksson, Johan; Svendsen, John Sigurd; Brandsdal, Bjørn Olav. Structural studies of small cationic antimicrobial peptides by NMR and computer simulations have facilitated a refined pharmacophore model able to predict activity versus Staphylococcus aureus. SMASH 2011 2011-09-18 - 2011-09-21 2011.
  • Isaksen, Geir Villy; Karstad, Rasmus Tørnes; Isaksson, Johan Mattias; Svenson, Johan; Svendsen, John Sigurd; Brandsdal, Bjørn Olav. Structure of short cationic antimicrobial peptides and their interactions with membranes as revealed by MD simulations. ISB Winter School 2010 2010-12-07 - 2010-12-10 2010.
  • Agback, Tatiana; Woestenenk, Esmeralda; Sahlberg, Christer; Isaksson, Johan; Kovacs, Helena. Selective labeling in large proteins as a tool for monitoring ligand-protein contacts by NMR. Part I: Proof of concept demonstrated on dUTPase complexes.. SMASH 2010 2010-09-26 - 2010-09-29 2010.
  • Isaksson, Johan; Flaten, Gøril Eide; Brandsdal, Bjørn Olav; Svendsen, John Sigurd. Studies of Small Synthetic Anti-microbial Peptides by NMR and Molecular Modeling. Joint EUROMAR 2010 + ISMAR 2010 2010-07-05 - 2010-07-09 2010.
  • Kovacs, Helena; Isaksson, Johan; Nyström, Susanne; Agback, Tatiana; Bertini, Ivano; Luchinat, Claudio. Determination of Water-Protein contacts by NMR. Application to the MMP-CGS27023A complex.. SMASH 2008 2008-09-07 - 2010-09-10 2008.
   Se alle arbeider i CRIStin



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